Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2010-6-25
pubmed:abstractText
Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus Gliocladium virens and to transform them to benomyl resistance with a Neurospora crassa beta-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of nuclear condition in vegetative and asexual structures demonstrated that no structure of G. virens is dependably uninucleate and thus preferentially suitable for transformation.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Oct
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3052-6
pubmed:dateRevised
2010-9-20
pubmed:year
1990
pubmed:articleTitle
Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance.
pubmed:affiliation
Biocontrol of Plant Diseases Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.
pubmed:publicationType
Journal Article