16348156

Source:http://linkedlifedata.com/resource/pubmed/id/16348156

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0017189, umls-concept:C0025663, umls-concept:C0032659, umls-concept:C0162358, umls-concept:C0205314, umls-concept:C0439148, umls-concept:C0521033, umls-concept:C0679622, umls-concept:C1707927, umls-concept:C1961019
pubmed:issue	4
pubmed:dateCreated	2010-6-25
pubmed:abstractText	An endpoint dilution procedure, based on the technique of most probable numbers, was developed to enumerate anaerobic chytridiomycetes as thallus-forming units. The method does not distinguish between zoospores and thalli, but does permit enumeration of fungal populations with respect to their ability to digest plant cell walls. Fibrolytic populations in batch culture, ruminal contents, and feces were compared by relating viable counts to the dry matter content of enumerated samples (i.e., thallus-forming units per gram of dry matter). Batch cultures of Neocallimastix sp. strain R1 grown on wheat straw were used to assess the enumeration procedure and to demonstrate the potential of the technique for quantification of anaerobic fungi in vivo. Determination of total ruminal contents from steers enabled the quantification of the entire population of fiber-degrading anaerobic fungi in the reticulorumen. The enumeration procedure revealed substantial populations of fibrolytic anaerobic fungi in fresh and air-dried feces. Populations in fresh feces were equivalent to those in ruminal contents, but declined exponentially with time in dry feces. Minimum values were obtained from dry feces 90 days after drying, and anaerobic fungi were detectable for up to 210 days thereafter.
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-1462, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-16345408, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-16345904, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-16347929, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-21939, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-3395688, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-3395690, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-3606103, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-3606104, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-4448982, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-533150, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-6275002, http://linkedlifedata.com/resource/pubmed/commentcorrection/16348156-853288
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7605801
pubmed:status	PubMed-not-MEDLINE
pubmed:month	Apr
pubmed:issn	0099-2240
pubmed:author	pubmed-author:BeeverD EDE, pubmed-author:GillMM, pubmed-author:King-SpoonerCC, pubmed-author:TheodorouM KMK
pubmed:issnType	Print
pubmed:volume	56
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1073-8
pubmed:dateRevised	2010-9-20
pubmed:year	1990
pubmed:articleTitle	Enumeration of anaerobic chytridiomycetes as thallus-forming units: novel method for quantification of fibrolytic fungal populations from the digestive tract ecosystem.
pubmed:affiliation	Agricultural and Food Research Council Institute for Grassland and Animal Production, Animal and Grassland Research Station, Hurley, Maidenhead, Berkshire SL6 5LR, United Kingdom.
pubmed:publicationType	Journal Article