Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-6-25
pubmed:abstractText
The ability of three strains of Lactobacillus acidophilus to survive and retain beta-galactosidase activity during storage in liquid nitrogen at -196 degrees C and during subsequent storage in milk at 5 degrees C was tested. The level of beta-galactosidase activity varied among the three strains (0.048 to 0.177 U/10 organisms). Freezing and storage at -196 degrees C had much less adverse influence on viability and activity of the enzyme than did storage in milk at 5 degrees C. The strains varied in the extent of the losses of viability and beta-galactosidase activity during both types of storage. There was not a significant interaction between storage at -196 degrees C and subsequent storage at 5 degrees C. The strains that exhibited the greatest losses of beta-galactosidase activity during storage in milk at 5 degrees C also exhibited the greatest losses in viability at 5 degrees C. However, the losses in viability were of much greater magnitude than were the losses of enzymatic activity. This indicates that some cells of L. acidophilus which failed to form colonies on the enumeration medium still possessed beta-galactosidase activity. Cultures of L. acidophilus to be used as dietary adjuncts to improve lactose utilization in humans should be carefully selected to ensure that adequate beta-galactosidase activity is provided.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Apr
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
898-902
pubmed:dateRevised
2010-9-20
pubmed:year
1988
pubmed:articleTitle
Influence of Storage at Freezing and Subsequent Refrigeration Temperatures on beta-Galactosidase Activity of Lactobacillus acidophilus.
pubmed:affiliation
Animal Science Department, Oklahoma State University, Stillwater, Oklahoma 74078-0425.
pubmed:publicationType
Journal Article