Source:http://linkedlifedata.com/resource/pubmed/id/16347312
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2010-6-25
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| pubmed:abstractText |
Two endoxylanases produced by C. acetobutylicum ATCC 824 were purified to homogeneity by column chromatography. Xylanase A, which has a molecular weight of 65,000, hydrolyzed larchwood xylan randomly, yielding xylohexaose, xylopentaose, xylotetraose, xylotriose, and xylobiose as end products. Xylanase B, which has a molecular weight of 29,000, also hydrolyzed xylan randomly, giving xylotriose and xylobiose as end products. Xylanase A hydrolyzed carboxymethyl cellulose with a higher specific activity than xylan. It also exhibited high activity on acid-swollen cellulose. Xylanase B showed practically no activity against either cellulose or carboxymethyl cellulose but was able to hydrolyze lichenan with a specific activity similar to that for xylan. Both xylanases had no aryl-beta-xylosidase activity. The smallest oligosaccharides degraded by xylanases A and B were xylohexaose and xylotetraose, respectively. The two xylanases demonstrated similar K(m) and V(max) values but had different pH optima and isoelectric points. Ouchterlony immunodiffusion tests showed that xylanases A and B lacked antigenic similarity.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-13578996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-16346847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-16346904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-16347313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-3653922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-4114358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-6509395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-7081984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16347312-782186
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
0099-2240
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
53
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
644-50
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| pubmed:dateRevised |
2010-9-20
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| pubmed:year |
1987
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| pubmed:articleTitle |
Purification and Characterization of Two Endoxylanases from Clostridium acetobutylicum ATCC 824.
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| pubmed:affiliation |
Department of Microbiology and Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
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| pubmed:publicationType |
Journal Article
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