Linked Life Data

16342976

Source:http://linkedlifedata.com/resource/pubmed/id/16342976

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
26
pubmed:dateCreated
2005-12-13
pubmed:abstractText
Dynamic light scattering (DLS) is extensively used for measuring macromolecule diffusion coefficients. Contrary to classical techniques based on macroscopic concentration gradients, DLS probes microscopic fluctuations in concentration. DLS accuracy and its concordance with macroscopic-gradient techniques remains an outstanding important issue. We measured lysozyme diffusion coefficients in aqueous salt using both DLS and Rayleigh interferometry, a highly accurate macroscopic-gradient technique. The precision of our results is unprecedented. We find that our DLS values were systematically 2% higher than interferometry values. We believe that our interferometric measurements have produced the most accurate diffusion data ever reported for a protein, providing a new standard for quality control of DLS measurements. Furthermore, by interferometry, we have determined the whole diffusion coefficient matrix required for rigorously describing lysozyme-salt coupled diffusion. For the first time, we experimentally demonstrate that DLS does not provide the protein diffusion coefficient but one eigenvalue of the diffusion coefficient matrix.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0743-7463
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12085-9
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Protein diffusion coefficients determined by macroscopic-gradient Rayleigh interferometry and dynamic light scattering.
pubmed:affiliation
Department of Chemistry, Texas Christian University, Fort Worth, Texas 76129, USA. o.annunziata@tcu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.