Linked Life Data

16341898

Source:http://linkedlifedata.com/resource/pubmed/id/16341898

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-2-10
pubmed:abstractText
In order to monitor the trans labilization of cisplatin at physiological pH we have prepared the complex cis-[PtCl(2)((13)CH(3)NH(2))(2)] and studied its interactions with excess glutathione in aqueous solution at neutral pH by two-dimensional [1H,13C] heteronuclear single-quantum correlation (HSQC) NMR spectroscopy. [1H,13C] HSQC spectroscopy is a good method for following the release of (13)CH(3)NH(2) but is not so good for characterizing the Pt species in solution. In the reaction of cisplatin with glutathione, Pt-S bonds are formed and Pt-NH(3) bonds are broken. The best technique for following the formation of Pt-S bonds of cisplatin is by UV spectroscopy. [1H,13C] HSQC spectroscopy is the best method for following the breaking of the Pt-N bonds. [1H,15N] HSQC spectroscopy is the best method for characterizing the different species in solution. However, the intensity of the peaks in the (15)NH(3)-Pt-S region, in [1H,15N] HSQC, reflects a balance between the formation of Pt-S bonds, which increases the signal intensity, and the trans labilization, which decreases the signal intensity. [1H,15N] HSQC spectroscopy and [1H,13C] HSQC spectroscopy are complementary techniques that should be used in conjunction in order to obtain the most accurate information on the interaction of platinum complexes with sulfur-containing ligands.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0949-8257
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-88
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
The trans labilization of cis-[PtCl2(13CH3NH2)2] by glutathione can be monitored at physiological pH by [1H,13C] HSQC NMR.
pubmed:affiliation
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University of Jerusalem, P.O. Box 12065, 91120 Jerusalem, Israel. gibson@md.huji.ac.il
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't