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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
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pubmed:dateCreated |
1992-8-27
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pubmed:abstractText |
We have constructed several hybrid human interleukin-6 (IL-6) variants in which the carboxyl-terminus, which includes a receptor binding site of IL-6 has been replaced with the C-terminus of various proteins homologous to human IL-6. IL-6 hybrids with the C-terminus of human growth hormone and human granulocyte-colony stimulating factor maintain part of the biological activity of human IL-6. Replacing the C-terminus of human IL-6 with the C-terminus of mouse and rat IL-6 resulted in a normal or increased activity on a mouse cell line; however, this gave a low (to 200-fold less) activity on a human cell line compared to wild-type human IL-6. We therefore conclude that the C-terminus of IL-6 plays an important role in the species specificity of IL-6.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
306
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
262-4
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1633883-Amino Acid Sequence,
pubmed-meshheading:1633883-Animals,
pubmed-meshheading:1633883-Base Sequence,
pubmed-meshheading:1633883-Cloning, Molecular,
pubmed-meshheading:1633883-DNA,
pubmed-meshheading:1633883-Humans,
pubmed-meshheading:1633883-Interleukin-6,
pubmed-meshheading:1633883-Mice,
pubmed-meshheading:1633883-Molecular Sequence Data,
pubmed-meshheading:1633883-Mutagenesis, Site-Directed,
pubmed-meshheading:1633883-Rats,
pubmed-meshheading:1633883-Sequence Alignment,
pubmed-meshheading:1633883-Species Specificity
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pubmed:year |
1992
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pubmed:articleTitle |
Construction and functional analysis of hybrid interleukin-6 variants. Characterization of the role of the C-terminus for species specificity.
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pubmed:affiliation |
University of North Carolina, Department of Pathology, Chapel Hill.
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pubmed:publicationType |
Journal Article
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