| pubmed-article:1633810 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1633810 | lifeskim:mentions | umls-concept:C0003732 | lld:lifeskim |
| pubmed-article:1633810 | lifeskim:mentions | umls-concept:C1069942 | lld:lifeskim |
| pubmed-article:1633810 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
| pubmed-article:1633810 | lifeskim:mentions | umls-concept:C0060672 | lld:lifeskim |
| pubmed-article:1633810 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1633810 | pubmed:dateCreated | 1992-8-25 | lld:pubmed |
| pubmed-article:1633810 | pubmed:abstractText | Methanobacterium wolfei is a thermophilic methanogenic archaeon which requires tungsten or molybdenum for growth. We have found that the organism contains two formylmethanofuran dehydrogenases, one of which is a tungsten enzyme. Indirect evidence indicates that the other formylmethanofuran dehydrogenase is a molybdenum enzyme. The tungsten enzyme was purified and characterized. The native enzyme had an apparent molecular mass of 130 kDa. SDS/PAGE revealed a composition of three subunits of apparent molecular mass 35, 51 and 64 kDa, the N-terminal amino acid sequences of two of which were determined. 0.3-0.4 mol tungsten/mol enzyme was found but no molybdenum. The pterin cofactor was identified as molybdopterin guanine dinucleotide. The purified enzyme exhibited a specific activity of 8.3 mumol.min-1.mg protein-1 and an apparent Km for formylmethanofuran and methylviologen of 13 microM and 0.4 mM, respectively. The optimum temperature for activity was 65 degrees C. At 40-60 degrees C, the rate increased with a Q10 of 1.9; the activation energy of the reaction was 45 kJ/mol. The enzyme was found to require potassium ions for thermostability. The oxygen-sensitive enzyme was not inactivated by cyanide. | lld:pubmed |
| pubmed-article:1633810 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1633810 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1633810 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1633810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1633810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1633810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1633810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1633810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1633810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1633810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1633810 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1633810 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1633810 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:1633810 | pubmed:author | pubmed-author:ThauerR KRK | lld:pubmed |
| pubmed-article:1633810 | pubmed:author | pubmed-author:LinderDD | lld:pubmed |
| pubmed-article:1633810 | pubmed:author | pubmed-author:RichterMM | lld:pubmed |
| pubmed-article:1633810 | pubmed:author | pubmed-author:SchmitzR ARA | lld:pubmed |
| pubmed-article:1633810 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1633810 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1633810 | pubmed:volume | 207 | lld:pubmed |
| pubmed-article:1633810 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1633810 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1633810 | pubmed:pagination | 559-65 | lld:pubmed |
| pubmed-article:1633810 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
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| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
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| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:meshHeading | pubmed-meshheading:1633810-... | lld:pubmed |
| pubmed-article:1633810 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1633810 | pubmed:articleTitle | A tungsten-containing active formylmethanofuran dehydrogenase in the thermophilic archaeon Methanobacterium wolfei. | lld:pubmed |
| pubmed-article:1633810 | pubmed:affiliation | Laboratorium für Mikrobiologie, Philipps-Universität Marburg, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:1633810 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1633810 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1633810 | lld:pubmed |
