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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2005-12-12
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pubmed:abstractText |
Phosphatidylinositol lipid signaling cascades are integral part of TCR-CD3 signaling. The mechanisms by which phosphatidylinositol kinases are coupled to TCR-CD3 complex remain elusive. Here we report an association of type II PtdIns 4-kinase with TCR-CD3 zeta chain upon cross-linking. Mapping studies have revealed that the C-terminal ITAM is critical for docking of the enzyme on the zeta chain. The association is shown to be tyrosyl phosphorylation dependent as mutation of Y-151 and Y-142 on the C-terminal ITAM disrupts interaction of the two proteins. Identification of the associated type II PtdIns 4-kinase revealed that the beta isoform of the enzyme interacts with the zeta chain in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Phosphatidylinositol 4-Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/antigen T cell receptor, zeta chain
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0161-5890
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
454-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16337488-1-Phosphatidylinositol 4-Kinase,
pubmed-meshheading:16337488-Amino Acid Motifs,
pubmed-meshheading:16337488-Antibodies, Monoclonal,
pubmed-meshheading:16337488-Enzyme Activation,
pubmed-meshheading:16337488-Humans,
pubmed-meshheading:16337488-Immunologic Capping,
pubmed-meshheading:16337488-Jurkat Cells,
pubmed-meshheading:16337488-Lymphocytes,
pubmed-meshheading:16337488-Membrane Proteins,
pubmed-meshheading:16337488-Mutagenesis, Site-Directed,
pubmed-meshheading:16337488-Phosphorylation,
pubmed-meshheading:16337488-Phosphotyrosine,
pubmed-meshheading:16337488-Protein Interaction Mapping,
pubmed-meshheading:16337488-Protein Processing, Post-Translational,
pubmed-meshheading:16337488-Receptors, Antigen, T-Cell,
pubmed-meshheading:16337488-Recombinant Fusion Proteins,
pubmed-meshheading:16337488-Transfection
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pubmed:year |
2006
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pubmed:articleTitle |
Type II phosphatidylinositol 4-kinase beta associates with TCR-CD3 zeta chain in Jurkat cells.
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pubmed:affiliation |
Biotechnology Group, School of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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