|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2005-12-21
|
|
pubmed:abstractText |
A method has been developed for rapidly separating and detecting strychnine and brucine using a poly(dimethysiloxane) (PDMS) microchip and electrochemical (EC) detection. PDMS microchannels dynamically modified by Brij35 are shown to be more efficient than native ones. The two analytes are well separated within 90 s in 70 mmol/L acetate buffer (pH 5.5) containing 0.01% (v/v) Brij35. Detection limits were found to be 1.0 micromol/L for strychnine and 0.2 micromol/L for brucine at S/N = 3. The method was used to determine trace strychnine and brucine in rat serum, and the results obtained correlate well with those obtained via high-performance liquid chromatography (HPLC).
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
1618-2642
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
384
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
265-70
|
|
pubmed:meshHeading |
pubmed-meshheading:16333604-Animals,
pubmed-meshheading:16333604-Buffers,
pubmed-meshheading:16333604-Dimethylpolysiloxanes,
pubmed-meshheading:16333604-Electrochemistry,
pubmed-meshheading:16333604-Hydrogen-Ion Concentration,
pubmed-meshheading:16333604-Microarray Analysis,
pubmed-meshheading:16333604-Molecular Structure,
pubmed-meshheading:16333604-Polyethylene Glycols,
pubmed-meshheading:16333604-Rats,
pubmed-meshheading:16333604-Reproducibility of Results,
pubmed-meshheading:16333604-Strychnine,
pubmed-meshheading:16333604-Time Factors
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Rapid separation of strychnine and brucine on a dynamically modified poly(dimethylsiloxane) microchip followed by electrochemical detection.
|
|
pubmed:affiliation |
Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, 210093, Nanjing, PR China.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
