Linked Life Data

16332279

Source:http://linkedlifedata.com/resource/pubmed/id/16332279

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-12-7
pubmed:abstractText
The responses of cells in the visual cortex to stimulation of the two eyes changes dramatically following a period of monocular visual deprivation (MD) during a critical period in early life. This phenomenon, referred to as ocular dominance (OD) plasticity, is a widespread model for understanding cortical plasticity. In this study, we designed stimulus patterns and quantification methods to analyze OD in the mouse visual cortex using optical imaging of intrinsic signals. Using periodically drifting bars restricted to the binocular portion of the visual field, we obtained cortical maps for both contralateral (C) and ipsilateral (I) eyes and computed OD maps as (C - I)/(C + I). We defined the OD index (ODI) for individual animals as the mean of the OD map. The ODI obtained from an imaging session of less than 30 min gives reliable measures of OD for both normal and monocularly deprived mice under Nembutal anesthesia. Surprisingly, urethane anesthesia, which yields excellent topographic maps, did not produce consistent OD findings. Normal Nembutal-anesthetized mice have positive ODI (0.22 +/- 0.01), confirming a contralateral bias in the binocular zone. For mice monocularly deprived during the critical period, the ODI of the cortex contralateral to the deprived eye shifted negatively towards the nondeprived, ipsilateral eye (ODI after 2-day MD: 0.12 +/- 0.02, 4-day: 0.03 +/- 0.03, and 6- to 7-day MD: -0.01 +/- 0.04). The ODI shift induced by 4-day MD appeared to be near maximal, consistent with previous findings using single-unit recordings. We have thus established optical imaging of intrinsic signals as a fast and reliable screening method to study OD plasticity in the mouse.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-10027290, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-10499792, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-10559430, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-10664805, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-10724170, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-12408850, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-12534981, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-12765606, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-12818182, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-12904462, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-15017002, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-3785405, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-5883732, http://linkedlifedata.com/resource/pubmed/commentcorrection/16332279-8627365
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0952-5238
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
685-91
pubmed:dateRevised
2011-9-14
pubmed:meshHeading
pubmed:articleTitle
Optical imaging of the intrinsic signal as a measure of cortical plasticity in the mouse.
pubmed:affiliation
W.M. Keck Foundation Center for Integrative Neuroscience, Department of Physiology, University of California, San Francisco, CA 94143-0444, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural