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rdf:type |
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lifeskim:mentions |
umls-concept:C0015576,
umls-concept:C0020792,
umls-concept:C0040649,
umls-concept:C0079904,
umls-concept:C0205314,
umls-concept:C0441655,
umls-concept:C0679622,
umls-concept:C0680022,
umls-concept:C1427381,
umls-concept:C1515877,
umls-concept:C1879547,
umls-concept:C1880022
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pubmed:issue |
4
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pubmed:dateCreated |
2005-12-5
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pubmed:databankReference |
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pubmed:abstractText |
The Ras family of small GTPases regulates a wide variety of cellular functions that include cell growth, differentiation, and transformation. In this study, we identified and characterized a novel member of Ras family named RHEBL1, belonging to the Rheb branch of small GTPase proteins. The cDNA sequence contains an open reading frame of 551 bp, encoding a putative protein of 183 amino acid residues. The expression pattern of RHEBL1 showed that it was ubiquitously expressed in 17 tissues. RHEBL1 gene encodes a 20.69 kDa protein, localized in cytoplasm when overexpressed in COS7 cells. Reporter gene assays showed that overexpression of RHEBL1 in HEK 293T cells strongly activated the transcriptional activities of NF-kappa B, while the mutant (D60K) only weakly activates NF-kappa B-mediated transcription. Our findings suggest that RHEBL1 is a positive regulator of NF-kappa B-mediated gene transcription.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0301-4851
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
205-14
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16328882-Amino Acid Sequence,
pubmed-meshheading:16328882-Animals,
pubmed-meshheading:16328882-Base Sequence,
pubmed-meshheading:16328882-Blotting, Western,
pubmed-meshheading:16328882-COS Cells,
pubmed-meshheading:16328882-Cercopithecus aethiops,
pubmed-meshheading:16328882-Cluster Analysis,
pubmed-meshheading:16328882-DNA, Complementary,
pubmed-meshheading:16328882-DNA Primers,
pubmed-meshheading:16328882-Gene Components,
pubmed-meshheading:16328882-Gene Expression,
pubmed-meshheading:16328882-Humans,
pubmed-meshheading:16328882-Molecular Sequence Data,
pubmed-meshheading:16328882-Mutagenesis,
pubmed-meshheading:16328882-NF-kappa B,
pubmed-meshheading:16328882-Phylogeny,
pubmed-meshheading:16328882-Sequence Alignment,
pubmed-meshheading:16328882-Sequence Analysis, DNA,
pubmed-meshheading:16328882-Transcriptional Activation,
pubmed-meshheading:16328882-ras Proteins
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pubmed:year |
2005
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pubmed:articleTitle |
Identification and characterization of RHEBL1, a novel member of Ras family, which activates transcriptional activities of NF-kappa B.
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pubmed:affiliation |
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai 200433, P.R. China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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