Source:http://linkedlifedata.com/resource/pubmed/id/16325952
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2005-12-26
|
| pubmed:abstractText |
Two cross-linkers based on polyethylene glycol (PEG) (MW=6 and 8 kDa), were synthesized for self-assembling and formation of nanoparticles of branched, high molecular weight polyethylenimine (PEI). Cross-linking was realized in two ways, viz., ionic as well as covalent. Ionic cross-linking was accomplished by using PEG-bis (phosphate) whereas, the covalent one was achieved by using PEG-bis (p-nitrophenylcarbonate). A range of nanoparticles of PEI was prepared by varying the degree of cross-linking (i.e. the amount of cross-linkers used). PEI-PEG nanoparticles were characterized by dynamic light scattering and transmission electron microscopy and found to be in the range of approximately 18-75 nm (hydrodynamic radii) with almost uniform population. Subsequently, these particles were used for DNA binding assay and zeta-potential measurements, taking native PEI-PEG nanoparticles as reference. As expected, the zeta potential values decreased, on increasing the percentage of cross-linking as well as on complexation with DNA. Further, PEI-PEG nanoparticles were investigated for their transfecting efficacy on COS-1 cells. It was found that PEI-PEG nanoparticles were 5- to 16-fold more efficient as transfecting agents compared to lipofectin and PEI itself. The toxicity of PEI-PEG nanoparticles was found to be reduced considerably in comparison to PEI polymer, as determined by MTT colorimetric assay. Out of the various systems prepared, PEI-PEG8000 (5% ionic) nanoparticles were found to be the most efficient transfecting agent for in vitro transfection.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Excipients,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethylene Glycols,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethyleneimine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0168-3659
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
110
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
457-68
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:16325952-Animals,
pubmed-meshheading:16325952-COS Cells,
pubmed-meshheading:16325952-Cell Survival,
pubmed-meshheading:16325952-Cells, Cultured,
pubmed-meshheading:16325952-Cercopithecus aethiops,
pubmed-meshheading:16325952-Cross-Linking Reagents,
pubmed-meshheading:16325952-DNA,
pubmed-meshheading:16325952-Electrochemistry,
pubmed-meshheading:16325952-Erythrocytes,
pubmed-meshheading:16325952-Excipients,
pubmed-meshheading:16325952-Hemolysis,
pubmed-meshheading:16325952-Humans,
pubmed-meshheading:16325952-Microscopy, Electron, Transmission,
pubmed-meshheading:16325952-Molecular Weight,
pubmed-meshheading:16325952-Nanostructures,
pubmed-meshheading:16325952-Particle Size,
pubmed-meshheading:16325952-Phosphates,
pubmed-meshheading:16325952-Polyethylene Glycols,
pubmed-meshheading:16325952-Polyethyleneimine,
pubmed-meshheading:16325952-Transfection
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Polyethylenimine nanoparticles as efficient transfecting agents for mammalian cells.
|
| pubmed:affiliation |
Nucleic Acids Research Laboratory and Division of Allergy and Infectious Diseases, Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi - 110 007, India.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|