Linked Life Data

16324157

Source:http://linkedlifedata.com/resource/pubmed/id/16324157

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2005-12-5
pubmed:abstractText
Nuclear localization of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is implicated in the process of apoptosis. To study the function of GAPDH, we expressed GAPDH C-terminally fused with or without nuclear localization signal (NLS) in SH-SY5Y and NB41A3 cells using a retrovirus expression system. GAPDH carrying NLS (GAPDH-NLS) was expressed mainly in the nucleus. However, expression of GAPDH-NLS did not cause any difference in cell survival rate as compared to that of the vector alone or GAPDH without NLS. Treatment with 1-Methyl-4-phenyl-pyridium iodide (MPP+) caused no difference in the cell survival rate or in the pattern or extent of apoptosis among the three transductants. In the cells expressing GAPDH without NLS, MPP+ did not cause visible translocation of GAPDH into nucleus before the onset of apoptosis. Since GAPDH is known to comprise a CRM1-mediated nuclear export signal, we blocked the nuclear export of GAPDH by treatment with leptomycin B, an inhibitor of CRM1-mediated nuclear export. The treatment did not cause any difference in apoptosis among the three transductants. An additional treatment with MPP+ induced no apoptotic difference in these cells. Thus, we have concluded that a simple nuclear localization of GAPDH does not induce apoptosis, and that MPP+-induced apoptosis is not caused by nuclear translocation of GAPDH.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1356-9597
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1211-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16324157-1-Methyl-4-phenylpyridinium, pubmed-meshheading:16324157-Active Transport, Cell Nucleus, pubmed-meshheading:16324157-Apoptosis, pubmed-meshheading:16324157-Cell Nucleus, pubmed-meshheading:16324157-Cell Survival, pubmed-meshheading:16324157-DNA Fragmentation, pubmed-meshheading:16324157-Dose-Response Relationship, Drug, pubmed-meshheading:16324157-Electron Transport Complex I, pubmed-meshheading:16324157-Enzyme Inhibitors, pubmed-meshheading:16324157-Fatty Acids, Unsaturated, pubmed-meshheading:16324157-Glyceraldehyde-3-Phosphate Dehydrogenases, pubmed-meshheading:16324157-Humans, pubmed-meshheading:16324157-Karyopherins, pubmed-meshheading:16324157-Kinetics, pubmed-meshheading:16324157-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:16324157-Recombinant Fusion Proteins
pubmed:year
2005
pubmed:articleTitle
Nuclear localization of glyceraldehyde-3-phosphate dehydrogenase is not involved in the initiation of apoptosis induced by 1-Methyl-4-phenyl-pyridium iodide (MPP+).
pubmed:affiliation
Department of Neurology, Institute of Advanced Medicine, Wakayama Medical University, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't