Linked Life Data

16323207

Source:http://linkedlifedata.com/resource/pubmed/id/16323207

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-12-27
pubmed:abstractText
The novel morphological characteristics of N9 microglial cells and primary cultured rat microglial cells were examined using the bitotin-IB4 and streptavidin-FITC system. Numerous fine, long processes of both microglial cell preparations formed a network, beginning after 30 min in culture. Dye coupling studies did not show communication between neighbouring cells via the processes in normal conditions. The network of microglial cell processes was well formed into a 'resting state' by 16-24 hr after re-plating. After being challenged by 3 mM ATP the microglial cells were activated, became amoeboid-like cells within 2 hr and finally floated in the culture medium. The complicated network of processes did not retract to the microglial cell body. Flow cytometry analysis showed that the majority of these floating cells were alive and could recover to the resting state after ATP was removed from the culture medium.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-101
pubmed:dateRevised
2007-8-13
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Microglial morphology and its transformation after challenge by extracellular ATP in vitro.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Second Military Medical University Shanghai, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't