Linked Life Data

16319119

Source:http://linkedlifedata.com/resource/pubmed/id/16319119

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-12-12
pubmed:abstractText
Most eggs in the animal kingdom establish a primary, animal-vegetal axis maternally, and specify the remaining two axes during development. In sea urchin embryos, the expression of Nodal on the oral (ventral) side of the embryo is the first known molecular determinant of the oral-aboral axis (the embryonic dorsoventral axis), and is crucial for specification of the oral territory. We show that p38 MAPK acts upstream of Nodal and is required for Nodal expression in the oral territory. p38 is uniformly activated early in development, but, for a short interval at late blastula stage, is asymmetrically inactivated in future aboral nuclei. Experiments show that this transient asymmetry of p38 activation corresponds temporally to both oral specification and the onset of oral Nodal expression. Uniform inhibition of p38 prevents Nodal expression and axis specification, resulting in aboralized embryos. Nodal and its target Gsc each rescue oral-aboral specification and patterning when expressed asymmetrically in p38-inhibited embryos. Thus, our results indicate that p38 is required for oral specification through its promotion of Nodal expression in the oral territory.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
133
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21-32
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16319119-Amino Acid Sequence, pubmed-meshheading:16319119-Animals, pubmed-meshheading:16319119-Blotting, Western, pubmed-meshheading:16319119-Body Patterning, pubmed-meshheading:16319119-Computational Biology, pubmed-meshheading:16319119-Gene Expression Regulation, Developmental, pubmed-meshheading:16319119-Gene Library, pubmed-meshheading:16319119-In Situ Hybridization, pubmed-meshheading:16319119-Models, Biological, pubmed-meshheading:16319119-Molecular Sequence Data, pubmed-meshheading:16319119-Mouth, pubmed-meshheading:16319119-Nodal Protein, pubmed-meshheading:16319119-Oligonucleotides, pubmed-meshheading:16319119-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16319119-Sea Urchins, pubmed-meshheading:16319119-Transforming Growth Factor beta, pubmed-meshheading:16319119-p38 Mitogen-Activated Protein Kinases
pubmed:year
2006
pubmed:articleTitle
p38 MAPK is essential for secondary axis specification and patterning in sea urchin embryos.
pubmed:affiliation
DCBM Group, Biology Department, Duke University, Durham, NC 27708, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural