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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-1-30
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pubmed:abstractText |
Stimulation of Jurkat T cells by high concentrations of concanavalin A (ConA) induced an elevation of the endogenous adenosine diphosphoribose (ADPR) concentration and an inward current significantly different from the Ca2+ release-activated Ca2+ current (I(CRAC)). Electrophysiological characterization and activation of a similar current by infusion of ADPR indicated that the ConA-induced current is carried by TRPM2. Expression of TRPM2 in the plasma membrane of Jurkat T cells was demonstrated by reverse transcription-PCR, Western blot, and immunofluorescence. Inhibition of ADPR formation reduced ConA-mediated, but not store-operated, Ca2+ entry and prevented ConA-induced cell death of Jurkat cells. Moreover, gene silencing of TRPM2 abolished the ADPR- and ConA-mediated inward current. Thus, ADPR is a novel second messenger significantly involved in ConA-mediated cell death in T cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:FliegertRalfR,
pubmed-author:GasserAndreasA,
pubmed-author:GlassmeierGünterG,
pubmed-author:GuseAndreas HAH,
pubmed-author:HeinDörteD,
pubmed-author:HeinerInkaI,
pubmed-author:KrügerSylviaS,
pubmed-author:LanghorstMatthias FMF,
pubmed-author:MeinkeStephanS,
pubmed-author:OppenheimerNormanN,
pubmed-author:SchwarzJürgen RJR,
pubmed-author:WeberKarinK
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2489-96
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pubmed:dateRevised |
2007-8-13
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pubmed:meshHeading |
pubmed-meshheading:16316998-Adenosine Diphosphate Ribose,
pubmed-meshheading:16316998-Calcium,
pubmed-meshheading:16316998-Cell Death,
pubmed-meshheading:16316998-Concanavalin A,
pubmed-meshheading:16316998-Electrophysiology,
pubmed-meshheading:16316998-Humans,
pubmed-meshheading:16316998-Jurkat Cells,
pubmed-meshheading:16316998-Lymphocyte Activation,
pubmed-meshheading:16316998-Second Messenger Systems,
pubmed-meshheading:16316998-T-Lymphocytes,
pubmed-meshheading:16316998-TRPM Cation Channels
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pubmed:year |
2006
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pubmed:articleTitle |
Activation of T cell calcium influx by the second messenger ADP-ribose.
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pubmed:affiliation |
University Medical Center Hamburg-Eppendorf, Center of Experimental Medicine, Institute of Biochemistry and Molecular Biology I: Cellular Signal Transduction.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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