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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2005-11-29
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pubmed:abstractText |
The mRNA processing body (P-body) is a cellular structure that regulates gene expression by degrading cytoplasmic mRNA. The objective of this study was to identify and characterize novel components of the mammalian P-body. Approximately 5% of patients with the autoimmune disease primary biliary cirrhosis have antibodies directed against this structure. Serum from one of these patients was used to identify a cDNA encoding Ge-1, a 1,401-amino-acid protein. Ge-1 contains an N-terminal WD 40 motif and C-terminal domains characterized by a repeating psi(X(2-3)) motif. Ge-1 co-localized with previously identified P-body components, including proteins involved in mRNA decapping (DCP1a and DCP2) and the autoantigen GW 182. The Ge-1 C-terminal domain was necessary and sufficient to target the protein to P-bodies. Following exposure of cells to oxidative stress, Ge-1-containing P-bodies were found adjacent to TIA-containing stress granules. During the recovery period, TIA returned to the nucleus while Ge-1-containing P-bodies localized to the perinuclear region. siRNA-mediated knock-down of Ge-1 resulted in loss of P-bodies containing Ge-1, DCP1a, and DCP2. In contrast, Ge-1-containing P-bodies persisted despite knock-down of DCP2. Taken together, the results of this study show that Ge-1 is a central component of P-bodies and suggest that Ge-1 may act prior to the 5(')-decapping step in mRNA degradation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-10322433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-10747033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-11751054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-11780629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-12440955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-12730603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-13130130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-14598044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-14749774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15067023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15152089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15189161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15340168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15494374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15625657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15731006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15742439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15840819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-15967811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-7520377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-8900092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-9067524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16314453-9592163
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/DAPI,
http://linkedlifedata.com/resource/pubmed/chemical/EDC4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Caps,
http://linkedlifedata.com/resource/pubmed/chemical/Serine
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1355-8382
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1795-802
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:16314453-Amino Acid Motifs,
pubmed-meshheading:16314453-Amino Acid Sequence,
pubmed-meshheading:16314453-Antibodies,
pubmed-meshheading:16314453-Autoantigens,
pubmed-meshheading:16314453-Cell Line, Tumor,
pubmed-meshheading:16314453-Cytoplasmic Structures,
pubmed-meshheading:16314453-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:16314453-Fluorescent Dyes,
pubmed-meshheading:16314453-Humans,
pubmed-meshheading:16314453-Immunohistochemistry,
pubmed-meshheading:16314453-Indoles,
pubmed-meshheading:16314453-Liver Cirrhosis, Biliary,
pubmed-meshheading:16314453-Nuclear Proteins,
pubmed-meshheading:16314453-Oxidative Stress,
pubmed-meshheading:16314453-Protein Structure, Tertiary,
pubmed-meshheading:16314453-Proteins,
pubmed-meshheading:16314453-RNA, Messenger,
pubmed-meshheading:16314453-RNA, Small Interfering,
pubmed-meshheading:16314453-RNA Caps,
pubmed-meshheading:16314453-Serine
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pubmed:year |
2005
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pubmed:articleTitle |
Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body.
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pubmed:affiliation |
Department of Medicine, Havard Medical School, the Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, Charlestown, MA 02129, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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