Source:http://linkedlifedata.com/resource/pubmed/id/16314102
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2005-12-12
|
| pubmed:abstractText |
We developed a gold coated glass chip bearing a poly(ethyleneglycol) (PEG) type compound as hydrophilic spacer for surface plasmon resonance studies, which enabled adequate estimation of K(d) value between FK506 and FKBP12 not only using purified FKBP12 (K(d)=22 nM) but also using Escherichia coli lysate expressing FKBP12 (K(d)=15 nM). These results indicated effectiveness of the PEG spacer for reduction of nonspecific interactions. Chemical stability and simple surface-structure of the novel chip are also attractive.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0968-0896
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
537-43
|
| pubmed:meshHeading |
pubmed-meshheading:16314102-Animals,
pubmed-meshheading:16314102-Brain,
pubmed-meshheading:16314102-Escherichia coli,
pubmed-meshheading:16314102-Gold,
pubmed-meshheading:16314102-Rats,
pubmed-meshheading:16314102-Surface Plasmon Resonance,
pubmed-meshheading:16314102-Surface Properties,
pubmed-meshheading:16314102-Tacrolimus,
pubmed-meshheading:16314102-Tacrolimus Binding Protein 1A
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Reduction of nonspecific binding proteins to self-assembled monolayer on gold surface.
|
| pubmed:affiliation |
Chemistry Department, Reverse Proteomics Research Institute Co. Ltd, 2-6-7 Kazusa-Kamatari, Chiba 292-0818, Japan.
|
| pubmed:publicationType |
Journal Article
|