Linked Life Data

16306061

Source:http://linkedlifedata.com/resource/pubmed/id/16306061

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-3-9
pubmed:abstractText
Elongation of rice internodes is one of the most important agronomic traits, which determines the plant height and underlies the grain yield. It has been shown that the elongation of internodes is under genetic control, and various factors are implicated in the process. Here, we report a detailed characterization of an elongated uppermost internode1 (eui1) mutant, which has been used in hybrid rice breeding. In the eui1-2 mutant, the cell lengths in the uppermost internodes are significantly longer than that of wild type and thus give rise to the elongated uppermost internode. It was found that the level of active gibberellin was elevated in the mutant, whereas its growth in response to gibberellin is similar to that of the wild type, suggesting that the higher level accumulation of gibberellin in the eui1 mutant causes the abnormal elongation of the uppermost internode. Consistently, the expression levels of several genes which encode gibberellin biosynthesis enzymes were altered. We cloned the EUI1 gene, which encodes a putative cytochrome P450 monooxygenase, by map-based cloning and found that EUI1 was weakly expressed in most tissues, but preferentially in young panicles. To confirm its function, transgenic experiments with different constructs of EUI1 were conducted. Overexpression of EUI1 gave rise to the gibberellin-deficient-like phenotypes, which could be partially reversed by supplementation with gibberellin. Furthermore, apart from the alteration of expression levels of the gibberellin biosynthesis genes, accumulation of SLR1 protein was found in the overexpressing transgenic plants, indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis. Therefore, we proposed that EUI1 plays a negative role in gibberellin-mediated regulation of cell elongation in the uppermost internode of rice.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0032-0781
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-91
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16306061-Amino Acid Sequence, pubmed-meshheading:16306061-Blotting, Western, pubmed-meshheading:16306061-Chromosomes, Plant, pubmed-meshheading:16306061-Cloning, Molecular, pubmed-meshheading:16306061-Cytochrome P-450 Enzyme System, pubmed-meshheading:16306061-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:16306061-Gene Expression Regulation, Plant, pubmed-meshheading:16306061-Genes, Plant, pubmed-meshheading:16306061-Gibberellins, pubmed-meshheading:16306061-Molecular Sequence Data, pubmed-meshheading:16306061-Mutation, pubmed-meshheading:16306061-Oryza sativa, pubmed-meshheading:16306061-Phenotype, pubmed-meshheading:16306061-Plant Shoots, pubmed-meshheading:16306061-Plants, Genetically Modified, pubmed-meshheading:16306061-RNA, Plant, pubmed-meshheading:16306061-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16306061-Signal Transduction
pubmed:year
2006
pubmed:articleTitle
EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internode elongation by modulating gibberellin responses in rice.
pubmed:affiliation
National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't