Source:http://linkedlifedata.com/resource/pubmed/id/16301638
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
2005-11-22
|
| pubmed:abstractText |
The role of IL-7 in lymphoid development and T cell homeostasis has been extensively documented. However, the role of IL-7 in human B cell development remains unclear. We used a xenogeneic human cord blood stem cell/murine stromal cell culture to study the development of CD19+ B-lineage cells expressing the IL-7R. CD34+ cord blood stem cells were cultured on the MS-5 murine stromal cell line supplemented with human G-CSF and stem cell factor. Following an initial expansion of myeloid/monocytoid cells within the initial 2 wk, CD19+/pre-BCR- pro-B cells emerged, of which 25-50% expressed the IL-7R. FACS-purified CD19+/IL-7R+ cells were larger and, when replated on MS-5, underwent a dose-dependent proliferative response to exogenous human IL-7 (0.01-10.0 ng/ml). Furthermore, STAT5 phosphorylation was induced by the same concentrations of human IL-7. CD19+/IL-7R- cells were smaller and did not proliferate on MS-5 after stimulation with IL-7. In a search for cytokines that promote human B cell development in the cord blood stem cell/MS-5 culture, we made the unexpected finding that murine IL-7 plays a role. Murine IL-7 was detected in MS-5 supernatants by ELISA, recombinant murine IL-7 induced STAT5 phosphorylation in CD19+/IL-7R+ pro-B cells and human B-lineage acute lymphoblastic leukemias, and neutralizing anti-murine IL-7 inhibited development of CD19+ cells in the cord blood stem cell/MS-5 culture. Our results support a model wherein IL-7 transduces a replicative signal to normal human B-lineage cells that is complemented by additional stromal cell-derived signals essential for normal human B cell development.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD19,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5 Transcription Factor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
175
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
7325-31
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:16301638-Animals,
pubmed-meshheading:16301638-Antigens, CD19,
pubmed-meshheading:16301638-Antigens, CD34,
pubmed-meshheading:16301638-B-Lymphocytes,
pubmed-meshheading:16301638-Cell Differentiation,
pubmed-meshheading:16301638-Cell Lineage,
pubmed-meshheading:16301638-Cell Proliferation,
pubmed-meshheading:16301638-Cells, Cultured,
pubmed-meshheading:16301638-Coculture Techniques,
pubmed-meshheading:16301638-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16301638-Flow Cytometry,
pubmed-meshheading:16301638-Hematopoietic Stem Cells,
pubmed-meshheading:16301638-Humans,
pubmed-meshheading:16301638-Interleukin-7,
pubmed-meshheading:16301638-Mice,
pubmed-meshheading:16301638-Models, Immunological,
pubmed-meshheading:16301638-Phosphorylation,
pubmed-meshheading:16301638-Receptors, Interleukin-7,
pubmed-meshheading:16301638-STAT5 Transcription Factor,
pubmed-meshheading:16301638-Stromal Cells
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.
|
| pubmed:affiliation |
The Cancer Center, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|