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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2005-12-14
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pubmed:abstractText |
Serine/arginine-rich (SR) proteins are important regulators of mRNA splicing. Several postsplicing activities have been described for a subset of shuttling SR proteins, including regulation of mRNA export and translation. Using the fibronectin gene to study the links between signal-transduction pathways and SR protein activity, we show that growth factors not only modify the alternative splicing pattern of the fibronectin gene but also alter translation of reporter messenger RNAs in an SR protein-dependent fashion, providing two coregulated levels of isoform-specific amplification. These effects are inhibited by specific small interfering RNAs against SR proteins and are mediated by the AKT kinase, which elicits opposite effects to those evoked by overexpressing SR protein kinases Clk and SRPK. These results show how SR protein activity is modified in response to extracellular stimulation, leading to a concerted regulation of splicing and translation.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/serine-arginine-rich splicing...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1545-9993
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pubmed:author |
pubmed-author:BlausteinMatíasM,
pubmed-author:CáceresJavier FJF,
pubmed-author:CosoOmar AOA,
pubmed-author:KornblihttAlberto RAR,
pubmed-author:MuñozManuel JMJ,
pubmed-author:MuschiettiJorge PJP,
pubmed-author:PelischFedericoF,
pubmed-author:QuadranaLeandroL,
pubmed-author:SanfordJeremy RJR,
pubmed-author:SrebrowAnabellaA,
pubmed-author:TanosTamaraT,
pubmed-author:WengierDiegoD
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1037-44
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:16299516-Amino Acid Sequence,
pubmed-meshheading:16299516-Animals,
pubmed-meshheading:16299516-Cell Nucleus,
pubmed-meshheading:16299516-Cytoplasm,
pubmed-meshheading:16299516-Fibronectins,
pubmed-meshheading:16299516-Growth Substances,
pubmed-meshheading:16299516-Humans,
pubmed-meshheading:16299516-Molecular Sequence Data,
pubmed-meshheading:16299516-Nuclear Proteins,
pubmed-meshheading:16299516-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:16299516-Phosphoproteins,
pubmed-meshheading:16299516-Protein Biosynthesis,
pubmed-meshheading:16299516-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:16299516-RNA, Messenger,
pubmed-meshheading:16299516-RNA, Small Interfering,
pubmed-meshheading:16299516-RNA Splicing,
pubmed-meshheading:16299516-RNA-Binding Proteins,
pubmed-meshheading:16299516-Signal Transduction
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pubmed:year |
2005
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pubmed:articleTitle |
Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT.
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pubmed:affiliation |
Instituto de Fisiología, Biología Molecular y Neurociencias, Consejo Nacional de Investigaciones Científicas y Técnicas, Departamento de Fisiología, Biología Molecular y Celular, Universidad de Buenos Aires, Ciudad Universitaria, Argentina.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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