Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-12-12
pubmed:databankReference
pubmed:abstractText
Degradation of lipid peroxides leads to the formation of cytotoxic 2-alkenals and oxenes (collectively designated reactive carbonyls). The novel NADPH-dependent oxidoreductase 2-alkenal reductase (AER; EC 1.3.1.74) from Arabidopsis (Arabidopsis thaliana), which is encoded by the gene At5g16970, catalyzes the reduction of the alpha,beta-unsaturated bond of reactive carbonyls, and hence is presumed to function in antioxidative defense in plants. Here we show that Arabidopsis AER (At-AER) has a broad substrate spectrum to biologically relevant reactive carbonyls. Besides 2-alkenals, the enzyme recognized as substrates the lipid peroxide-derived oxenes 9-oxo-octadeca-(10E),(12Z)-dienoic acid and 13-oxo-octadeca-(9E),(11Z)-dienoic acid, as well as the potent genotoxin 4-oxo-(2E)-nonenal, altogether suggesting AER has a key role in the detoxification of reactive carbonyls. To validate this conclusion by in vivo studies, transgenic tobacco (Nicotiana tabacum) plants that had 100- to 250-fold higher AER activity levels than control plants were generated. The engineered plants exhibited significantly less damage from either (1) the exogenously administered 4-hydroxy-(2E)-nonenal, (2) treatment with methyl viologen plus light, or (3) intense light. We further show that the At-AER protein fused with the Aequorea victoria green fluorescent protein localizes in cytosol and the nucleus in Bright-Yellow 2 cells. These results indicate that reactive carbonyls mediate photooxidative injury in leaf cells, and At-AER in the cytosol protects the cells by reducing the alpha,beta-unsaturated bond of the photoproduced reactive carbonyls.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-10103020, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-10848984, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-10956056, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11033416, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11115125, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11115128, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11181709, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11259420, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11289602, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11408659, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11524419, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11553744, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-11849595, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-12098696, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-12102510, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-12122207, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-12199705, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-12689622, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-12706498, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-12904208, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-15175756, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-15388247, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-15652504, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-1937131, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-7479844, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-7592828, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-7840616, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-8429010, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-8454610, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-8524341, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-8605288, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-8643495, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-9175718, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-9473298, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-9556565, http://linkedlifedata.com/resource/pubmed/commentcorrection/16299173-9632657
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1773-83
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Protection against photooxidative injury of tobacco leaves by 2-alkenal reductase. Detoxication of lipid peroxide-derived reactive carbonyls.
pubmed:affiliation
Science Research Center , Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8515, Japan. mano@yamaguchi-u.ac.jp
pubmed:publicationType
Journal Article