Linked Life Data

16298384

Source:http://linkedlifedata.com/resource/pubmed/id/16298384

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-3-27
pubmed:abstractText
Structural modifications of AOT/water/isooctane reverse micelles due to incorporation of proteins were studied at various water contents and protein concentrations, using small-angle X-ray scattering (SAXS) experiments under static conditions, rheometry analysis, and SR-SAXS experiments under induced shear flow. Two proteins, lysozyme (pI 11.1, Mw 14,300 Da) and BSA (pI 4.3, Mw 66,700 Da), were chosen as models. SAXS analysis of protein-containing reverse micelles at low water content detected minima in the average micelle size versus protein concentration curve, for both proteins, below and above their isoelectric point. This minimum was attributed to changes in the size distribution of the reverse micelles. SAXS measurements of reverse micelles at high water content have shown them to have a cylindrical form. Incorporation of lysozyme at pH 7 into the cylindrical micelles induced a shape transition to spherical micelles, which was associated with a decreased viscosity. SR-SAXS measurements under induced shear flow and dynamic conditions revealed alignment of the cylindrical micelles in the flow direction. The anisotropy parameter, a measure of the degree of the spatial order, was found to increase with increasing shear rate and to decrease with increasing lysozyme concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9797
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
297
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
276-83
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Structure modifications of AOT reverse micelles due to protein incorporation.
pubmed:affiliation
Department of Chemical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't