|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2006-4-3
|
|
pubmed:abstractText |
Homodimeric bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor beta superfamily that has been used for bone grafting. We were interested in exploring the functions of BMP-2 in other disease areas and focused on expressing and purifying active BMP-2 proteins. We have developed a new approach which involves using FoldIt refolding buffer to refold BMP-2 followed by a heparin affinity column to separate correctly folded dimer from monomer. A high yield of 29.4 mg BMP-2 dimer per gram cell wet weight was achieved. The purified BMP-2 dimer was shown to possess the same level of activity as BMP-2 from CHO cells as tested by the induction of alkaline phosphatase activity in C2C12 cells. This approach has potential application in refolding and purifying other homodimeric proteins.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
1046-5928
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
46
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
374-8
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:16298141-Animals,
pubmed-meshheading:16298141-Bone Morphogenetic Protein 2,
pubmed-meshheading:16298141-Bone Morphogenetic Proteins,
pubmed-meshheading:16298141-CHO Cells,
pubmed-meshheading:16298141-Cricetinae,
pubmed-meshheading:16298141-Cricetulus,
pubmed-meshheading:16298141-Escherichia coli,
pubmed-meshheading:16298141-Gene Expression,
pubmed-meshheading:16298141-Humans,
pubmed-meshheading:16298141-Inclusion Bodies,
pubmed-meshheading:16298141-Protein Folding,
pubmed-meshheading:16298141-Recombinant Proteins,
pubmed-meshheading:16298141-Transforming Growth Factor beta
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Expression, purification, and renaturation of bone morphogenetic protein-2 from Escherichia coli.
|
|
pubmed:affiliation |
Immusol, Inc., 10790 Roselle Street, San diego, CA 92121, USA. long@immusol.com
|
|
pubmed:publicationType |
Journal Article
|
