Linked Life Data

16298077

Source:http://linkedlifedata.com/resource/pubmed/id/16298077

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-1-9
pubmed:abstractText
The hypothesis that damage to mitochondrial DNA by reactive oxygen species increases the activity of nuclear and mitochondrial transcription factors for mitochondrial DNA replication was tested in the in vivo rat brain. Mitochondrial reactive oxygen species generation was stimulated using pre-convulsive doses of hyperbaric oxygen and hippocampal mitochondrial DNA content and neuronal and mitochondrial morphology and cell proliferation were evaluated at 1, 5 and 10 days. Gene expression was subsequently evaluated to assess nuclear and mitochondrial-encoded respiratory genes, mitochondrial transcription factor A, and nuclear respiratory transcription factors-1 and -2. After 1 day, a mitochondrial DNA deletion emerged involving Complex I and IV subunit-encoding regions that was independent of overt neurological or cytological O(2) toxicity, and resolved before the onset of cell proliferation. This damage was attenuated by blockade of neuronal nitric oxide synthase. Compensatory responses were found in nuclear gene expression for manganese superoxide dismutase, mitochondrial transcription factor A, and nuclear respiratory transcription factor-2. Enhanced nuclear respiratory transcription factor-2 binding activity in hippocampus was accompanied by a nearly three-fold boost in mitochondrial DNA content over 5 days. The finding that O(2) activates regional mitochondrial DNA transcription, replication, and mitochondrial biogenesis in the hippocampus may have important implications for maintaining neuronal viability after brain injury.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
493-504
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16298077-Animals, pubmed-meshheading:16298077-Cell Shape, pubmed-meshheading:16298077-DNA, Mitochondrial, pubmed-meshheading:16298077-DNA Replication, pubmed-meshheading:16298077-Electron Transport, pubmed-meshheading:16298077-GA-Binding Protein Transcription Factor, pubmed-meshheading:16298077-Gene Deletion, pubmed-meshheading:16298077-Gene Expression Regulation, Enzymologic, pubmed-meshheading:16298077-Hippocampus, pubmed-meshheading:16298077-Male, pubmed-meshheading:16298077-Mitochondria, pubmed-meshheading:16298077-Neurons, pubmed-meshheading:16298077-Nitric Oxide Synthase Type I, pubmed-meshheading:16298077-Nuclear Respiratory Factor 1, pubmed-meshheading:16298077-Oxidative Stress, pubmed-meshheading:16298077-Oxygen, pubmed-meshheading:16298077-Rats, pubmed-meshheading:16298077-Rats, Sprague-Dawley, pubmed-meshheading:16298077-Reactive Oxygen Species, pubmed-meshheading:16298077-Transcription Factors, pubmed-meshheading:16298077-Transcriptional Activation, pubmed-meshheading:16298077-Up-Regulation
pubmed:year
2006
pubmed:articleTitle
Oxygen-induced mitochondrial biogenesis in the rat hippocampus.
pubmed:affiliation
Department of Medicine and Anesthesiology and Center for Hyperbaric Medicine and Environmental Physiology, Duke University Medical Center, Box 3315, Durham, NC 27710, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural