Source:http://linkedlifedata.com/resource/pubmed/id/16298005
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2006-1-23
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| pubmed:abstractText |
Neurons become photosensitive by genetically introducing one of green algae-derived protein, channelrhodopsin-2 (ChR2). Here, we quantitatively investigated the rapidness of the light-gated current of ChR2 expressed in PC12 cells using blue light-emitting diode (LED) light. The light-gated current consists of two components, inactivating and non-inactivating. The magnitude of inactivating component was almost linearly related to the light intensity. The non-inactivating component showed a tendency to saturate at high illumination. Both the activation and inactivation rates of the light-gated current were linearly dependent on the light intensity. However, the activation rate (turning-on rate) is about 10-fold faster than the inactivation rate. Although the turning-off time constant was little dependent on the light intensity, that at the end of 1s light pulse was about two-fold larger than that at 20 ms. Neurons are also made photosensitive by the expression of ChR2 in the living animal. Since both the turning-on and turning-off time constants of light-gated current was smaller than the membrane time constant of neurons, the LED light illumination of the photosensitive neurons was enough to evoke action potentials in a pulse-to-pulse manner in an acute slice of hippocampus.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0168-0102
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
54
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
85-94
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:16298005-Algorithms,
pubmed-meshheading:16298005-Animals,
pubmed-meshheading:16298005-Chlorophyta,
pubmed-meshheading:16298005-DNA, Complementary,
pubmed-meshheading:16298005-Genetic Engineering,
pubmed-meshheading:16298005-Hippocampus,
pubmed-meshheading:16298005-Ion Channel Gating,
pubmed-meshheading:16298005-Ion Channels,
pubmed-meshheading:16298005-Kinetics,
pubmed-meshheading:16298005-Light,
pubmed-meshheading:16298005-Membrane Potentials,
pubmed-meshheading:16298005-Neurons,
pubmed-meshheading:16298005-PC12 Cells,
pubmed-meshheading:16298005-Patch-Clamp Techniques,
pubmed-meshheading:16298005-Photic Stimulation,
pubmed-meshheading:16298005-Photochemistry,
pubmed-meshheading:16298005-Rats,
pubmed-meshheading:16298005-Rhodopsin,
pubmed-meshheading:16298005-Sindbis Virus,
pubmed-meshheading:16298005-Transfection
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Kinetic evaluation of photosensitivity in genetically engineered neurons expressing green algae light-gated channels.
|
| pubmed:affiliation |
Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, 2-1 Seiryo-machi, Sendai 980-8575, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|