Linked Life Data

16297876

Source:http://linkedlifedata.com/resource/pubmed/id/16297876

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-11-29
pubmed:abstractText
Our previous study found that PKCalpha was highly expressed in the poor-differentiated human HCC cells and associated with cell migration and invasion. In this study, we further investigated the gene regulation of this enzyme. We showed that PKCalpha expression enhancement in the poor-differentiated human HCC cells was found neither by DNA amplification nor by increasing mRNA stability using differential PCR and mRNA decay assays. After screening seven transcription factors in the putative cis-acting regulatory elements of human PKCalpha promoters, only Elk-1 and MZF-1 antisense oligonucleotide showed a significant reduction in the PKCalpha mRNA level. They also reduced cell proliferation, cell migratory and invasive capabilities, and DNA binding activities in the PKCalpha promoter region. Over-expression assay confirmed that the PKCalpha expression may be modulated by these two factors at the transcriptional level. Therefore, these results may provide a novel mechanism for PKCalpha expression regulation in human HCC cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
339
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-25
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16297876-Cell Line, Tumor, pubmed-meshheading:16297876-Cell Movement, pubmed-meshheading:16297876-Cell Proliferation, pubmed-meshheading:16297876-DNA-Binding Proteins, pubmed-meshheading:16297876-Gene Amplification, pubmed-meshheading:16297876-Gene Expression Regulation, Enzymologic, pubmed-meshheading:16297876-Humans, pubmed-meshheading:16297876-Kruppel-Like Transcription Factors, pubmed-meshheading:16297876-Neoplasm Invasiveness, pubmed-meshheading:16297876-Oligodeoxyribonucleotides, Antisense, pubmed-meshheading:16297876-Promoter Regions, Genetic, pubmed-meshheading:16297876-Protein Kinase C-alpha, pubmed-meshheading:16297876-RNA, Messenger, pubmed-meshheading:16297876-RNA Stability, pubmed-meshheading:16297876-Transcription Factors, pubmed-meshheading:16297876-ets-Domain Protein Elk-1
pubmed:year
2006
pubmed:articleTitle
PKCalpha expression regulated by Elk-1 and MZF-1 in human HCC cells.
pubmed:affiliation
Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't