Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-12-30
pubmed:abstractText
Smooth muscle cells (SMCs) contain numerous calcium release domains, grouped into regions discharging as a single unit. Laser scanning confocal microscopy, voltage clamp and immunocytochemistry of single SMCs from small mesenteric arteries of guinea-pig were used to study the localisation, function and macromolecular composition of such calcium discharge regions (CDRs). Use of the Ca(2+)-sensitive fluorescent dye fluo-3 or fluo-4 with BODIPY TR-X ryanodine (BTR), a fluorescent derivative of ryanodine, showed spontaneous Ca(2+) sparks originating from regions stained by BTR, located immediately under the plasma membrane, in the arch formed by the sarcoplasmic reticulum surrounding the nucleus. Membrane depolarisation or application of noradrenaline or alpha,beta-methylene ATP, a P2X purinoceptor agonist, elicited Ca(2+) sparks from the same, spontaneous Ca(2+) spark-discharging region. The most active (primary) CDR accounted for nearly 60% of spontaneous transient outward currents at -40 mV and these were of significantly higher amplitude than the ones discharged by secondary CDRs. Immunocytochemical staining for type 1 IP(3) receptors, BK(Ca) channels, P2X(1) purinoceptors or alpha(1) adrenoceptors revealed their juxtaposition with BTR staining at the location typical of the primary CDR. These data suggest the existence of a primary calcium discharge region in SMCs; its position can be predicted from the cell's structure, it acts as a key region for the regulation of membrane potential via Ca(2+) sparks and is a potential link between the external, neurohumoral and the cell's internal, calcium signalling system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/4,4-difluoro-4-bora-3a,4a-diaza-s-in..., http://linkedlifedata.com/resource/pubmed/chemical/Boron Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes, http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate..., http://linkedlifedata.com/resource/pubmed/chemical/Large-Conductance..., http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, alpha-1, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X, http://linkedlifedata.com/resource/pubmed/chemical/Ryanodine, http://linkedlifedata.com/resource/pubmed/chemical/Ryanodine Receptor Calcium Release...
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0143-4160
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-29
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:16297446-Animals, pubmed-meshheading:16297446-Boron Compounds, pubmed-meshheading:16297446-Calcium Channels, pubmed-meshheading:16297446-Calcium Signaling, pubmed-meshheading:16297446-Fluorescent Dyes, pubmed-meshheading:16297446-Guinea Pigs, pubmed-meshheading:16297446-Inositol 1,4,5-Trisphosphate Receptors, pubmed-meshheading:16297446-Large-Conductance Calcium-Activated Potassium Channels, pubmed-meshheading:16297446-Male, pubmed-meshheading:16297446-Membrane Potentials, pubmed-meshheading:16297446-Mesenteric Arteries, pubmed-meshheading:16297446-Microscopy, Confocal, pubmed-meshheading:16297446-Muscle, Smooth, Vascular, pubmed-meshheading:16297446-Myocytes, Smooth Muscle, pubmed-meshheading:16297446-Receptors, Adrenergic, alpha-1, pubmed-meshheading:16297446-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:16297446-Receptors, Purinergic P2, pubmed-meshheading:16297446-Receptors, Purinergic P2X, pubmed-meshheading:16297446-Ryanodine, pubmed-meshheading:16297446-Ryanodine Receptor Calcium Release Channel
pubmed:year
2006
pubmed:articleTitle
Localisation, function and composition of primary Ca(2+) spark discharge region in isolated smooth muscle cells from guinea-pig mesenteric arteries.
pubmed:affiliation
Division of Basic Medical Sciences, Ion Channels and Cell Signalling Centre St. George's, University of London, Cranmer Terrace, SW17 0RE London, United Kingdom. v.pucovsky@sgul.ac.uk
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't