Linked Life Data

16292809

Source:http://linkedlifedata.com/resource/pubmed/id/16292809

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2005-11-18
pubmed:abstractText
[structure: see text] Carbohydrates play an important role in life processes, and combinatorial chemistry can provide useful sources of thousands of synthetic carbohydrates as potential ligands for biological receptors. To accelerate the detection of positive hits arising from specific interactions between a carbohydrate and a protein, the use of fluorescent dyes can serve as a reliable detecting tool. A study of labeled carbohydrates to lectins conjugated to a solid-support shows that succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X) dye provides by far the lowest level of nonspecific interaction with immobilized protein. This observation is in stark contrast with the commonly used labeling reagents constituted of charged and aromatic groups, for instance, FITC and TAMRA dyes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3263
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9809-13
pubmed:dateRevised
2010-2-12
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Investigation of nonspecific effects of different dyes in the screening of labeled carbohydrates against immobilized proteins.
pubmed:affiliation
Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't