Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2005-11-18
pubmed:abstractText
The twin-arginine translocation (Tat) pathway is present in a wide variety of prokaryotes and is capable of exporting partially or fully folded proteins from the cytoplasm. Although diverse classes of proteins are transported via the Tat pathway, in most organisms it facilitates the secretion of a relatively small number of substrates compared to the Sec pathway. However, computational evidence suggests that haloarchaea route nearly all secreted proteins to the Tat pathway. We have expanded previous computational analyses of the haloarchaeal Tat pathway and initiated in vivo characterization of the Tat machinery in a model haloarchaeon, Haloferax volcanii. Consistent with the predicted usage of the this pathway in the haloarchaea, we determined that three of the four identified tat genes in Haloferax volcanii are essential for viability when grown aerobically in complex medium. This represents the first report of an organism that requires the Tat pathway for viability when grown under such conditions. Deletion of the nonessential gene had no effect on the secretion of a verified substrate of the Tat pathway. The two TatA paralogs TatAo and TatAt were detected in both the membrane and cytoplasm and could be copurified from the latter fraction. Using size exclusion chromatography to further characterize cytoplasmic and membrane TatA proteins, we find these proteins present in high-molecular-weight complexes in both cellular fractions.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-10593889, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-10939242, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-11016950, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-11422364, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-11931160, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12029389, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12034867, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12180915, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12427925, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12533451, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12533452, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12562823, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12721369, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-12867413, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-14580344, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-14766575, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-14975527, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-15049806, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-15520287, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-15554971, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-15557327, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-15670849, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-2147413, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-8063095, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-8510664, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-8939424, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-9546395, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-9649434, http://linkedlifedata.com/resource/pubmed/commentcorrection/16291683-9783223
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
187
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8104-13
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Genetic and biochemical analysis of the twin-arginine translocation pathway in halophilic archaea.
pubmed:affiliation
Department of Biology, University of Pennsylvania, Philadelphia, 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural