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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2005-12-26
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pubmed:abstractText |
We have previously isolated anti-FcepsilonRIalpha autoantibodies from phage libraries of healthy donors and urticaria patients. Strikingly, the same antibody, LTMalpha15, was isolated from both libraries. Sequence analysis revealed a germline configuration of the LTMalpha15 variable heavy (V(H)) chain with a slightly mutated variable light (V(L)) chain supporting its classification as a natural autoantibody. Distribution analysis of anti-FcepsilonRIalpha autoantibodies by functional or serological tests delivered conflicting data. For this reason we have developed a new real-time PCR to analyse the distribution of LTMalpha15V(H) in healthy donors and urticaria patients. Our new bioinformatic program permitted the design of a minor groove binder (MGB) TaqMan probe that specifically detected the LTMalpha15V(H). We were able to demonstrate a broad range of rearranged V(H) gene copy number without any correlation to the state of health. Monitoring LTMalpha15V(H) gene copy number in a single donor over a period of 70 days revealed a time-related fluctuation of circulating B cells carrying LTMalpha15V(H). We propose that our real-time PCR may serve as a model for the quantification of natural antibody sequences at a monoclonal level.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1759
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
307
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
107-17
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16289529-Autoantibodies,
pubmed-meshheading:16289529-B-Lymphocytes,
pubmed-meshheading:16289529-Base Sequence,
pubmed-meshheading:16289529-Complementarity Determining Regions,
pubmed-meshheading:16289529-Computational Biology,
pubmed-meshheading:16289529-DNA Primers,
pubmed-meshheading:16289529-DNA Probes,
pubmed-meshheading:16289529-Gene Dosage,
pubmed-meshheading:16289529-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:16289529-Humans,
pubmed-meshheading:16289529-Immunoglobulin Heavy Chains,
pubmed-meshheading:16289529-Immunoglobulin Variable Region,
pubmed-meshheading:16289529-Molecular Sequence Data,
pubmed-meshheading:16289529-Polymerase Chain Reaction,
pubmed-meshheading:16289529-Receptors, IgE,
pubmed-meshheading:16289529-Sequence Analysis, DNA,
pubmed-meshheading:16289529-Time Factors,
pubmed-meshheading:16289529-Urticaria
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pubmed:year |
2005
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pubmed:articleTitle |
Detection of one VH antibody sequence in both healthy donors and urticaria patients.
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pubmed:affiliation |
Institute of Immunology, University of Bern, Inselspital, Switzerland.
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pubmed:publicationType |
Journal Article,
Comparative Study
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