Linked Life Data

16289409

Source:http://linkedlifedata.com/resource/pubmed/id/16289409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2006-2-14
pubmed:abstractText
Flavivirus NS2B-NS3 proteases are associated with neurovirulence, becoming an important target for insight into the virus-induced pathogenesis. In this study, a phage-displayed human brain cDNA library was used to detect possible interaction between brain proteins and the Japanese encephalitis virus (JEV) NS2B-NS3 protease. After six rounds of biopanning, eight high-affinity NS2B-NS3 protease-interacting phages were identified. Identified NS2B-NS3 protease-interacting brain proteins contained several repeats of the consensus motifs E(R/K)(R/K)K and G(R/K)(R/K) with the dibasic residues, being similar to the conserved cleavage sites among flavivirus proteases. In addition, three identified brain proteins (phage-24, 34, and 44) were predicted as the domain of trypsin inhibitor and basic region leucine zipper (bZIP) using the SMART genome search. Immunoprecipitation and cleavage of two brain fusion proteins (phage-24 and phage-46) by the NS2B-NS3 protease confirmed the specific interaction between identified brain proteins and the JEV NS2B-NS3 protease. Fluorogenic peptide substrate assays revealed dose-manner inhibitory effects of these two brain fusion proteins on the trans-cleavage activity of NS2B-NS3 protease. Moreover, in vitro signaling pathway assay revealed that the JEV NS2B-NS3 protease significantly inhibited the signaling pathway of activator protein 1(AP1), a member of the bZIP family. Our results provide an insight into the protein interaction network of the JEV NS2B-NS3 protease in human brain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0168-1702
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
106-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16289409-Amino Acid Sequence, pubmed-meshheading:16289409-Animals, pubmed-meshheading:16289409-Cercopithecus aethiops, pubmed-meshheading:16289409-Gene Library, pubmed-meshheading:16289409-Humans, pubmed-meshheading:16289409-Immunoprecipitation, pubmed-meshheading:16289409-Leucine Zippers, pubmed-meshheading:16289409-Molecular Sequence Data, pubmed-meshheading:16289409-Nerve Tissue Proteins, pubmed-meshheading:16289409-Peptide Library, pubmed-meshheading:16289409-Protein Binding, pubmed-meshheading:16289409-RNA Helicases, pubmed-meshheading:16289409-Serine Endopeptidases, pubmed-meshheading:16289409-Signal Transduction, pubmed-meshheading:16289409-Transcription Factor AP-1, pubmed-meshheading:16289409-Trypsin Inhibitors, pubmed-meshheading:16289409-Vero Cells, pubmed-meshheading:16289409-Viral Nonstructural Proteins
pubmed:year
2006
pubmed:articleTitle
Japanese encephalitis virus NS2B-NS3 protease binding to phage-displayed human brain proteins with the domain of trypsin inhibitor and basic region leucine zipper.
pubmed:affiliation
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan, Taiwan, ROC. cwlin@mail.cmu.edu.tw
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't