Linked Life Data

16280327

Source:http://linkedlifedata.com/resource/pubmed/id/16280327

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-1-12
pubmed:abstractText
Bcl10 overexpression and nuclear translocation were originally identified in mucosa-associated lymphoid tissue lymphoma with t(1;14)(p32;q32) chromosome translocation. DNA amplification of Bcl10 was also found in other solid tumors. We have recently shown that nuclear translocation of Bcl10 is a specific molecular determinant of Helicobacter pylori-independent mucosa-associated lymphoid tissue lymphoma (Kuo, S.-H., Chen, L. T., Yeh, K.-H., Wu, M. S., Hsu, H. C., Yeh, P. Y., Mao, T. L., Chen, C. L., Doong, S. L., Lin, J. T., and Cheng, A.-L. (2004) J. Clin. Oncol. 22, 3491-3497). However, the molecular mechanism of Bcl10 nuclear translocation remains unknown. In this study, we observed that tumor necrosis factor-alpha (TNFalpha) up-regulates the expression of Bcl10 and induces a fraction of Bcl10 nuclear translocation in human breast carcinoma MCF7 cells. Chromatin immunoprecipitation assays and electrophoretic mobility shift assays indicated that an NF-kappaB-binding site resides in the Bcl10 5 '-untranslated region. This study also demonstrates that Akt1, activated by TNFalpha, phosphorylates Bcl10 at Ser218 and Ser231 and that phosphorylated Bcl10 subsequently complexes with Bcl3 to enter the nucleus. Either inhibition of Akt1 or depletion of Bcl3 blocks Bcl10 nuclear translocation. In summary, these findings characterize a molecular linkage that directs Bcl10 nuclear translocation in response to TNFalpha treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
281
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-75
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16280327-Active Transport, Cell Nucleus, pubmed-meshheading:16280327-Adaptor Proteins, Signal Transducing, pubmed-meshheading:16280327-Breast Neoplasms, pubmed-meshheading:16280327-Cell Line, Tumor, pubmed-meshheading:16280327-Cell Nucleus, pubmed-meshheading:16280327-Gene Expression Regulation, Neoplastic, pubmed-meshheading:16280327-Humans, pubmed-meshheading:16280327-NF-kappa B, pubmed-meshheading:16280327-Phosphorylation, pubmed-meshheading:16280327-Protein Structure, Tertiary, pubmed-meshheading:16280327-Proto-Oncogene Proteins, pubmed-meshheading:16280327-Proto-Oncogene Proteins c-akt, pubmed-meshheading:16280327-Serine, pubmed-meshheading:16280327-Transcription Factors, pubmed-meshheading:16280327-Transcriptional Activation, pubmed-meshheading:16280327-Tumor Necrosis Factor-alpha, pubmed-meshheading:16280327-Up-Regulation
pubmed:year
2006
pubmed:articleTitle
A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus.
pubmed:affiliation
Cancer Research Center, College of Medicine, National Taiwan University, Taipei.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't