Linked Life Data

16279195

Source:http://linkedlifedata.com/resource/pubmed/id/16279195

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-11-10
pubmed:abstractText
This study describes the cloning and function of promoter pagC (P(pagC)) from Salmonella typhimurium. The expression plasmid containing the in vivo-inducible promoter pagC and HGV-NS3 gene was introduced into the attenuated Salmonella typhimurium SL7207 to investigate the function of P(pagC). The expressed HGV-NS3 protein was detectable by SDS-PAGE and Western blotting in the recombinant bacteria in the presence of low concentration of Mg2+ (<50 mmol/L). When the concentration of Mg2+ reached to 50 mmol/L, the amount of expressed HGV-NS3 was decreased significantly. The recombinant bacteria were multiplied in LB medium containing 50 mmol/L of Mg2+ and used as a DNA vaccine to orally inoculate C57 mice for three times. The results of serum antibodies, T lymphocyte proliferative response and cytotoxic T lymphocyte response of immunized mice showed that the oral vaccine could iuduce strong humoral and cellular immune responses in mice, which indicates that the P(pagC) is a strong in vivo-inducible promoter and can be used in attenuated Salmonella typhimurium to construct an effective oral vaccine.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0001-6209
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
308-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
[Cloning and functional analysis of promoter pagC from attenuated Salmonella typhimurium].
pubmed:affiliation
Department of Microbiology, Second Military Medical University, Shanghai 200433, China. smmuwhu@yahoo.com.cn
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't