Source:http://linkedlifedata.com/resource/pubmed/id/16277602
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2005-12-20
|
| pubmed:abstractText |
D-serine is now considered to be an endogenous co-agonist of the NMDA receptor in mammalian brain. To obtain insight into the molecular mechanisms underlying D-serine metabolism and function, we explored transcripts that are responsive to D-serine in the neocortex of the 8-day-old infant rat by a differential cloning technique, RNA arbitrarily primed PCR. We isolated a novel D-serine inducible transcript, D-serine-responsive transcript-2 (dsr-2), that was exclusively expressed in the brain. Sequence analysis of the corresponding cDNAs to the transcript revealed that the dsr-2 mRNA consists of 7199 nucleotides with an open reading frame encoding 111 amino acids. The dsr-2 gene was located on the reverse strand within an intron of the neurexin-3alpha gene, mapped to rat chromosome 6q24-31. The regional distribution of the basal expression of dsr-2 and its ontogenic changes in the brain closely correlated with those of free D-serine and of NMDA receptor R2B subunit mRNA, but were somewhat different from those of the neurexin-3alpha transcript. These findings suggest that dsr-2 may be involved in D-serine metabolism and/or function, and in the interactions between D-serine, NMDA receptor and neurexin-3alpha, in mammalian brain.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
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| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
95
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1541-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16277602-Adenosine Triphosphatases,
pubmed-meshheading:16277602-Animals,
pubmed-meshheading:16277602-Base Sequence,
pubmed-meshheading:16277602-Blotting, Northern,
pubmed-meshheading:16277602-Blotting, Southern,
pubmed-meshheading:16277602-Central Nervous System,
pubmed-meshheading:16277602-Cloning, Molecular,
pubmed-meshheading:16277602-DNA Fingerprinting,
pubmed-meshheading:16277602-Gene Expression Regulation,
pubmed-meshheading:16277602-Male,
pubmed-meshheading:16277602-Molecular Sequence Data,
pubmed-meshheading:16277602-Neocortex,
pubmed-meshheading:16277602-Nerve Tissue Proteins,
pubmed-meshheading:16277602-Prosencephalon,
pubmed-meshheading:16277602-RNA,
pubmed-meshheading:16277602-Rats,
pubmed-meshheading:16277602-Rats, Wistar,
pubmed-meshheading:16277602-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16277602-Serine,
pubmed-meshheading:16277602-Tissue Distribution
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Cloning of a D-serine-regulated transcript dsr-2 from rat cerebral neocortex.
|
| pubmed:affiliation |
Section of Psychiatry and Behavioral Sciences, Tokyo Medical and Dental University Graduate School, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|