Linked Life Data

16276903

Source:http://linkedlifedata.com/resource/pubmed/id/16276903

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-11-9
pubmed:abstractText
Labelled F-actin with FITC-phalloidin, we observed F-actin rearrangements by Streptococcus pneumoniae adhesion of type II pneumocytes (A549). Invasion of S. pneumoniae to A549 cells was determined by pretreating A549 cells with cytochalasin D. To investigate whether F-actin rearrangements can be blocked by Ca2+ inhibitors, A549 cells were pretreated with Ca2+ inhibitors datrollene. A549 cells were loaded with Fura-2/AM to determine the concentration of cytosolic free calcium by S. pneumoniae adhesion of A549 cells after 30,60,90 minutes respectively. Intact S. pneumoniae can promote F-actin rearrangements. Cytochalasin D is able to prevent S. pneumoniae invasion of A49 cells. S. pneumoniae adhesion of A549 cells increased cytosolic free calcium after 30, 60, 90 minuts. Ca2+ inhibitors datrollene block F-actin rearrangements dose dependently. It suggested S. pneumoniae can provoke F-actin rearrangements through Ca2+ signaling pathways, which will further lead to S. pneumoniae invasion of A49 cells.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0001-6209
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
788-92
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
[Ca2+ signaling pathways associated with the invasion of Streptococcus pneumoniae to type II pneumocytes].
pubmed:affiliation
Department of Clinical Biochemistry, Faculty of Laboratory Medicine, Chongqing University of Medical sciences, Chongqing 400016, China. yibingyin@21cn.com
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't