Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0017355,
umls-concept:C0079772,
umls-concept:C0185117,
umls-concept:C0376452,
umls-concept:C0596901,
umls-concept:C1314939,
umls-concept:C1412709,
umls-concept:C1514468,
umls-concept:C1517945,
umls-concept:C1527240,
umls-concept:C1883709,
umls-concept:C2911684
|
pubmed:issue |
3
|
pubmed:dateCreated |
1992-8-18
|
pubmed:abstractText |
We have isolated an H-2 deficient cell line (LR.4) from the T-cell lymphoma L5178Y which grew without restrictions in the peritoneal cavity of different inbred strains of mice. The use of polyclonal anti-H-2 antiserum and complement indicated that LR.4 cells did not express class I determinants on the cell membrane. Southern blots of genomic DNA of LR.4 cells showed that B2m genes were severely damaged and that class I H-2 genes were extensively methylated. Consequently, LR.4 cells failed to transcribe mRNAs for both B2m and class I H-2 genes. On the other hand, specific immunity to LR.4 was demonstrated in C57BL/6J mice since, in subsequent challenges with either LR.4 or EL4.4, LR.4 did not grow, whereas EL4.4 grew and killed the mice. In C57BL/6J mice, rejection of LR.4 was accompanied by the production of cytotoxic antibodies. The immune response induced in C57BL/6J mice was determined by non-H-2 antigenic determinants in LR.4 cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0960-7420
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
19
|
pubmed:geneSymbol |
B2m
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
141-58
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1627535-Animals,
pubmed-meshheading:1627535-Cell Division,
pubmed-meshheading:1627535-Cell Line,
pubmed-meshheading:1627535-Cell Membrane,
pubmed-meshheading:1627535-DNA, Neoplasm,
pubmed-meshheading:1627535-Genes, MHC Class I,
pubmed-meshheading:1627535-Leukemia L5178,
pubmed-meshheading:1627535-Methylation,
pubmed-meshheading:1627535-Mice,
pubmed-meshheading:1627535-Mice, Inbred Strains,
pubmed-meshheading:1627535-Mutation,
pubmed-meshheading:1627535-Species Specificity,
pubmed-meshheading:1627535-Transcription, Genetic
|
pubmed:year |
1992
|
pubmed:articleTitle |
Damage in B2m genes and DNA methylation of H-2 genes are involved in loss of expression of class I MHC products on the membrane of LR.4, a cell line derivative of the T-cell lymphoma L5178Y.
|
pubmed:affiliation |
Departamento de Inmunologia, Universidad Nacional Autonoma de Mexico.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|