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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2005-11-8
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pubmed:abstractText |
Using a newly generated monoclonal antibody (2E6) against human B7-H3, we explored the expression of the molecule on dendritic cells derived from monocytes (Mo-DCs). Its expression was examined by means of immunostaining and flow cytometric (FCM) analysis. The results showed that B7-H3 was expressed in the course of Mo-DC maturation induced with interleukin 4 (IL-4) and granulocyte/macrophage colony-stimulating factor (GM-CSF). The expression could be detected at all the stages of Mo-DC differentiation, and remained at a quite stable level. Interestingly, B7-H3 was not expressed by T cells and B cells, even these cells were activated respectively by PHA or PWM. A weak expression could be detected on resting monocytes. These data showed that constitutive expression of B7-H3 at a high level was found on imDCs and mDCs derived from monocytes. Due to no expression on T cells and B cells, we speculate that B7-H3 might be another valuable molecule marker for Mo-DCs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/B7 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Cd276 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
2042-0226
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
307-11
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16274630-Animals,
pubmed-meshheading:16274630-Antibodies, Monoclonal,
pubmed-meshheading:16274630-Antigens, CD80,
pubmed-meshheading:16274630-Antigens, Differentiation,
pubmed-meshheading:16274630-B-Lymphocytes,
pubmed-meshheading:16274630-B7 Antigens,
pubmed-meshheading:16274630-Cell Differentiation,
pubmed-meshheading:16274630-Cell Separation,
pubmed-meshheading:16274630-Dendritic Cells,
pubmed-meshheading:16274630-Flow Cytometry,
pubmed-meshheading:16274630-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:16274630-Humans,
pubmed-meshheading:16274630-Interleukin-4,
pubmed-meshheading:16274630-Mice,
pubmed-meshheading:16274630-Mice, Inbred BALB C,
pubmed-meshheading:16274630-Monocytes,
pubmed-meshheading:16274630-Recombinant Proteins,
pubmed-meshheading:16274630-T-Lymphocytes
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pubmed:year |
2005
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pubmed:articleTitle |
B7-H3: another molecule marker for Mo-DCs?
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pubmed:affiliation |
Clinical Immunology Laboratory of Jiangsu Province, Suzhou University No.1 Affiliated Hospital, Suzhou 215006, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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