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rdf:type |
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lifeskim:mentions |
umls-concept:C0021024,
umls-concept:C0034790,
umls-concept:C0039194,
umls-concept:C0040113,
umls-concept:C0242568,
umls-concept:C0332281,
umls-concept:C0457083,
umls-concept:C0458003,
umls-concept:C0678723,
umls-concept:C1334310,
umls-concept:C1424685,
umls-concept:C1602245,
umls-concept:C1706701,
umls-concept:C1708435
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pubmed:issue |
10
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pubmed:dateCreated |
2005-11-7
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pubmed:abstractText |
Among Ag-inexperienced naive T cells, the CD1d-restricted NKT cell that uses invariant TCR-alpha-chain is the most widely studied cell capable of prompt IL-4 inducibility. We show in this study that thymus CD161-CD44lowCD4+CD8- T cells promptly produce IL-4 upon TCR stimulation, a response that displays biased Vbeta(2/7/8) and Valpha3.2 TCR usage. The association of Vbeta family bias and IL-4 inducibility in thymus CD161-CD44lowCD4+CD8- T cells is found for B6, B10, BALB/c, CBA, B10.A(4R), and ICR mouse strains. Despite reduced IL-4 inducibility, there is a similarly biased Vbeta(2/7/8) TCR usage by IL-4 inducibility+ spleen CD161-CD44lowCD4+CD8- T cells. Removal of alpha-galacotosylceramide/CD1d-binding cells from CD161-CD44lowCD4+CD8- thymocytes does not significantly affect their IL-4 inducibility. The development of thymus CD161-CD44lowCD4+CD8- T cells endowed with IL-4 inducibility and their associated use of Vbeta(2/7/8) are beta2-microglobulin-, CD1d-, and p59fyn-independent. Thymus CD161-CD44lowCD4+CD8- T cells produce low and no IFN-gamma inducibility in response to TCR stimulation and to IL-12 + IL-18, respectively, and they express diverse complementarity determining region 3 sequences for both TCR-alpha- and -beta-chains. Taken together, these results demonstrate the existence of a NKT cell distinct, TCR-repertoire diverse naive CD4+ T cell subset capable of prompt IL-4 inducibility. This subset has the potential to participate in immune response to a relatively large number of Ags. The more prevalent nature of this unique T cell subset in the thymus than the periphery implies roles it might play in intrathymic T cell development and may provide a framework upon which mechanisms of developmentally regulated IL-4 gene inducibility can be studied.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1d,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Complementarity Determining Regions,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Galactosylceramides,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-galactosylceramide
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6537-50
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16272308-Amino Acid Sequence,
pubmed-meshheading:16272308-Animals,
pubmed-meshheading:16272308-Antigens, CD1,
pubmed-meshheading:16272308-Antigens, CD1d,
pubmed-meshheading:16272308-Antigens, Surface,
pubmed-meshheading:16272308-Base Sequence,
pubmed-meshheading:16272308-CD4-Positive T-Lymphocytes,
pubmed-meshheading:16272308-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16272308-Cell Differentiation,
pubmed-meshheading:16272308-Complementarity Determining Regions,
pubmed-meshheading:16272308-DNA,
pubmed-meshheading:16272308-Galactosylceramides,
pubmed-meshheading:16272308-Gene Expression Regulation,
pubmed-meshheading:16272308-Interleukin-4,
pubmed-meshheading:16272308-Interleukins,
pubmed-meshheading:16272308-Killer Cells, Natural,
pubmed-meshheading:16272308-Lectins, C-Type,
pubmed-meshheading:16272308-Mice,
pubmed-meshheading:16272308-Mice, Inbred BALB C,
pubmed-meshheading:16272308-Mice, Inbred CBA,
pubmed-meshheading:16272308-Mice, Inbred ICR,
pubmed-meshheading:16272308-Mice, Transgenic,
pubmed-meshheading:16272308-Molecular Sequence Data,
pubmed-meshheading:16272308-NK Cell Lectin-Like Receptor Subfamily B,
pubmed-meshheading:16272308-RNA, Messenger,
pubmed-meshheading:16272308-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:16272308-T-Lymphocyte Subsets
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pubmed:year |
2005
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pubmed:articleTitle |
CD1d-independent developmental acquisition of prompt IL-4 gene inducibility in thymus CD161(NK1)-CD44lowCD4+CD8- T cells is associated with complementarity determining region 3-diverse and biased Vbeta2/Vbeta7/Vbeta8/Valpha3.2 T cell receptor usage.
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pubmed:affiliation |
Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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