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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-1-23
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pubmed:abstractText |
We recently showed that both replicating and resting cells cultivated with ganciclovir (GCV) were killed when challenged with vesicular stomatitis virus G glycoprotein pseudotyped HIV-1-based virus-like particles (VLPs) carrying the Nef7 (i.e., an HIV-1 Nef mutant incorporating in virions at high levels)/herpes simplex virus-1 thymidine kinase (HSV-TK) fusion product. On this basis, a novel anti-HIV therapeutic approach based on Nef7/TK VLPs expressing X4 or R5 HIV cell receptor complexes has been attempted. We here report that (CD4-CXCR4) and (CD4-CCR5) Nef7-based VLPs efficiently enter cells infected by X4- or R5-tropic HIV-1 strains, respectively. Importantly, the delivery of the VLP-associated Nef7/TK led to cell death upon GCV treatment. Of interest, VLPs were effective also against non-replicating, HIV-1-infected primary human monocyte-derived macrophages. HIV-targeted VLPs represent a promising candidate for the treatment of persistently HIV-1-infected cells that are part of virus reservoirs resistant to HAART therapies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-HIV Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Ganciclovir,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, nef,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR5,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, HIV,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Virosomes,
http://linkedlifedata.com/resource/pubmed/chemical/nef Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
345
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
115-26
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16271741-Adult,
pubmed-meshheading:16271741-Anti-HIV Agents,
pubmed-meshheading:16271741-Antigens, CD4,
pubmed-meshheading:16271741-Blotting, Western,
pubmed-meshheading:16271741-Cell Death,
pubmed-meshheading:16271741-Cell Line,
pubmed-meshheading:16271741-Flow Cytometry,
pubmed-meshheading:16271741-Ganciclovir,
pubmed-meshheading:16271741-Gene Products, nef,
pubmed-meshheading:16271741-Genes, Reporter,
pubmed-meshheading:16271741-Green Fluorescent Proteins,
pubmed-meshheading:16271741-HIV-1,
pubmed-meshheading:16271741-Humans,
pubmed-meshheading:16271741-Male,
pubmed-meshheading:16271741-Proteins,
pubmed-meshheading:16271741-Receptors, CCR5,
pubmed-meshheading:16271741-Receptors, CXCR4,
pubmed-meshheading:16271741-Receptors, HIV,
pubmed-meshheading:16271741-Recombinant Fusion Proteins,
pubmed-meshheading:16271741-Thymidine Kinase,
pubmed-meshheading:16271741-Viral Proteins,
pubmed-meshheading:16271741-Virosomes,
pubmed-meshheading:16271741-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2006
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pubmed:articleTitle |
Selective elimination of HIV-1-infected cells by Env-directed, HIV-1-based virus-like particles.
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pubmed:affiliation |
AIDS Center, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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