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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-4-20
pubmed:abstractText
We examined the immunofluorescence of keratins 13 (K13) and 14 (K14) and differential interference contrast (DIC) images during the morphogenesis of filiform papillae and the keratinization of the lingual epithelium of rats on semi-ultrathin sections of epoxy resin-embedded samples by laser-scanning microscopy. We also examined semi-ultrathin sections of epoxy resin embedded, toluidine blue stained samples by light microscopy to obtain details of cell histology and morphology. No immunoreactivity specific for K13 and K14 was detected on the lingual epithelium of foetuses on days 13, 15 and 17 after conception (E13, E15 and E17), during which time the number of layers of cuboidal cells in the lingual epithelium increased from one to several. Immunoreactivity specific for K13 and K14 was first detected on the lingual epithelium of foetuses on E19. The immunoreactivity specific for K13 appeared in the suprabasal cells of the papillary and interpapillary cell columns and immunoreactivity specific for K14 was detected in the basal and suprabasal cells of the papillary and interpapillary cell columns. The lingual epithelium was composed of stratified squamous cells. The rudiments of filiform papillae were compactly arranged and interpapillary cell columns were very narrow. Filiform papillae developed gradually from postnatal day 0 (PO) to 21 (P21). The width of interpapillary spaces also increased during this period. Immunoreactivity specific for K13 and K14 was distinct at all postnatal stages examined. Thus, the patterns of immunoreactivity of K13 and K14 differed as the filiform papillae developed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-9969
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
416-26
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Immunohistochemical expression of keratins 13 and 14 in the lingual epithelium of rats during the morphogenesis of filiform papillae.
pubmed:affiliation
Advanced Research Center, The Nippon Dental University School of Dentistry at Niigata, 1-8 Hamaura-cho, Niigata 951-8580, Japan. iwasaki@ngt.ndu.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't