Linked Life Data

16268157

Source:http://linkedlifedata.com/resource/pubmed/id/16268157

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2005-11-4
pubmed:abstractText
TheChgH-GFP strain of the teleost medaka contains a regulatory region of the estrogen-responsive choriogenin H (chgH) gene fussed to the green fluorescent protein (GFP) gene. The strain was developed for the identification of environmental estrogens by noninvasive analysis of GFP fluorescence. In the present study, a quantification method for GFP by image analysis was establishedand applied to the analysis of time- and concentration-dependent GFP fluorescence in juvenile fish. Concentration-response analyses were performed with fish exposed for 14 d to 17beta-estradiol (0.37-367 pM), genistein (0.37-367 nM), or p-nonylphenol (0.367-1,835 nM). By means of image analysis, it was shown that ChgH-GFP was induced at 183.5 pM or greater 17beta-estradiol. Time-course and recovery experiments indicated a strong accumulation of GFP in the liver. Results of reverse transcriptase-polymerase chain reaction analysis of choriogenin H and vitellogenin demonstrated induction of gene expression for the same rangeof concentrations as that for GFP analysis. Neither expression of these genes nor GFP fluorescence was induced by genistein and p-nonylphenol. Although the ChgH-GFP strain failed to detect these weakly estrogenic compounds, the simplicity of the GFP quantification during early life stages of fish offers promising possibilities for further developments of transgenic strains using different target regulatory sequences.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0730-7268
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2553-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16268157-Animals, pubmed-meshheading:16268157-Animals, Genetically Modified, pubmed-meshheading:16268157-Antineoplastic Agents, pubmed-meshheading:16268157-Biological Markers, pubmed-meshheading:16268157-Dose-Response Relationship, Drug, pubmed-meshheading:16268157-Estradiol, pubmed-meshheading:16268157-Gene Expression Profiling, pubmed-meshheading:16268157-Genistein, pubmed-meshheading:16268157-Green Fluorescent Proteins, pubmed-meshheading:16268157-Larva, pubmed-meshheading:16268157-Liver, pubmed-meshheading:16268157-Oryzias, pubmed-meshheading:16268157-Phenols, pubmed-meshheading:16268157-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16268157-Tissue Distribution, pubmed-meshheading:16268157-Toxicity Tests, pubmed-meshheading:16268157-Vitellogenins
pubmed:year
2005
pubmed:articleTitle
Analysis of estrogenic effects by quantification of green fluorescent protein in juvenile fish of a transgenic medaka.
pubmed:affiliation
UFZ Centre for Environmental Research Leipzig-Halle in the Helmholtz-Association, Department of Cell Toxicology, D-04318 Leipzig, Germany. stefan.scholz@ufz.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't