Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2005-11-3
pubmed:abstractText
Androgen ablation therapies are effective in controlling prostate cancer. Although most cancers relapse and progress despite androgen ablation, some patients experience antiandrogen withdrawal syndrome, in which those treated with antiandrogen show clinical improvement when antiandrogen is discontinued. Although the androgen receptor (AR) is suggested to play an important role in prostate cancer progression even after the androgen ablation, limited tissue availability for molecular studies and small numbers of human prostate cancer cell lines have restricted prostate cancer research. Here, we describe KUCaP, a novel serially transplantable human prostate cancer xenograft model. We established KUCaP from liver metastatic tissue of a patient treated with antiandrogen bicalutamide. KUCaP expressed the AR with a point mutation at amino acid 741 (tryptophan to cysteine; W741C) in the ligand-binding domain. This mutation was also present in cancerous tissue used for generation of KUCaP. Although the growth of KUCaP in male mice was androgen dependent, bicalutamide aberrantly promoted the growth and prostate-specific antigen production of KUCaP. For the first time, we show the agonistic effect of bicalutamide to a xenograft with clinically induced AR mutation. This bicalutamide-responsive mutant AR will serve in the development of new therapies for androgen ablation-resistant prostate cancers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9611-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16266977-Androgen Antagonists, pubmed-meshheading:16266977-Anilides, pubmed-meshheading:16266977-Animals, pubmed-meshheading:16266977-Antineoplastic Agents, pubmed-meshheading:16266977-Cell Growth Processes, pubmed-meshheading:16266977-Exons, pubmed-meshheading:16266977-Humans, pubmed-meshheading:16266977-Liver Neoplasms, pubmed-meshheading:16266977-Male, pubmed-meshheading:16266977-Mice, pubmed-meshheading:16266977-Mice, SCID, pubmed-meshheading:16266977-Middle Aged, pubmed-meshheading:16266977-Neoplasm Transplantation, pubmed-meshheading:16266977-Neoplasms, Hormone-Dependent, pubmed-meshheading:16266977-Nitriles, pubmed-meshheading:16266977-Prostatic Neoplasms, pubmed-meshheading:16266977-Receptors, Androgen, pubmed-meshheading:16266977-Tosyl Compounds, pubmed-meshheading:16266977-Transplantation, Heterologous
pubmed:year
2005
pubmed:articleTitle
Antiandrogen bicalutamide promotes tumor growth in a novel androgen-dependent prostate cancer xenograft model derived from a bicalutamide-treated patient.
pubmed:affiliation
Department of Urology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't