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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
422
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pubmed:dateCreated |
2005-11-24
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pubmed:abstractText |
The Arabidopsis thaliana ascorbate-deficient vtc-1 mutant has only 30% ascorbate contents of the wild type (WT). This ascorbate-deficient mutant was used here to study the physiological roles of ascorbate under salt stress in vivo. Salt stress resulted in a more significant decrease in CO2 assimilatory capacity in the vtc-1 mutant than in the WT. Photosystem II function in the Arabidopsis vtc-1 mutant also showed an increased sensitivity to salt stress. Oxidative stress, indicated by the hydrogen peroxide content, increased more dramatically in the vtc-1 mutant than in the WT under salt stress. To clarify the reason for the increased oxidative stress in the vtc-1 mutant, the contents of small antioxidant compounds and the activities of several antioxidant enzymes in the ascorbate-glutathione cycle were measured. Despite an elevated glutathione pool in the vtc-1 mutant, the ascorbate contents and the reduced form of ascorbate decreased very rapidly under salt stress. These results showed that the activities of MDAR and DHAR were lower in the vtc-1 mutant than in the WT under salt stress. Thus, low intrinsic ascorbate and an impaired ascorbate-glutathione cycle in the vtc-1 mutant under salt stress probably induced a dramatic decrease in the reduced form of ascorbate, which resulted in both enhanced ROS contents and decreased NPQ in the vtc-1 mutant.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ascorbic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorophyll,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Mutant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Photosynthetic Reaction Center...,
http://linkedlifedata.com/resource/pubmed/chemical/Photosystem II Protein Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Water,
http://linkedlifedata.com/resource/pubmed/chemical/mannose 1-phosphate...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-0957
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3041-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16263910-Antioxidants,
pubmed-meshheading:16263910-Arabidopsis,
pubmed-meshheading:16263910-Arabidopsis Proteins,
pubmed-meshheading:16263910-Ascorbic Acid,
pubmed-meshheading:16263910-Carbon Dioxide,
pubmed-meshheading:16263910-Chlorophyll,
pubmed-meshheading:16263910-Glutathione,
pubmed-meshheading:16263910-Hydrogen Peroxide,
pubmed-meshheading:16263910-Mutant Proteins,
pubmed-meshheading:16263910-Nucleotidyltransferases,
pubmed-meshheading:16263910-Oxidation-Reduction,
pubmed-meshheading:16263910-Oxidative Stress,
pubmed-meshheading:16263910-Photosynthetic Reaction Center Complex Proteins,
pubmed-meshheading:16263910-Photosystem II Protein Complex,
pubmed-meshheading:16263910-Reactive Oxygen Species,
pubmed-meshheading:16263910-Sodium Chloride,
pubmed-meshheading:16263910-Water
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pubmed:year |
2005
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pubmed:articleTitle |
Increased sensitivity to salt stress in an ascorbate-deficient Arabidopsis mutant.
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pubmed:affiliation |
Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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