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rdf:type |
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lifeskim:mentions |
umls-concept:C0011306,
umls-concept:C0017278,
umls-concept:C0019704,
umls-concept:C0035696,
umls-concept:C0039194,
umls-concept:C0328767,
umls-concept:C0439859,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C1948023,
umls-concept:C2700640
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pubmed:issue |
5
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pubmed:dateCreated |
2006-2-22
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pubmed:abstractText |
Infection with human immunodeficiency virus type 1 (HIV-1) is characterized by dysfunction of HIV-1-specific T cells. To control the virus, antigen-loaded dendritic cells (DCs) might be useful to boost and broaden HIV-specific T-cell responses. In the present study, monocyte-derived DCs from nontreated HIV-1-seropositive patients were electroporated with codon-optimized ("humanized") mRNA encoding consensus HxB-2 (hHXB-2) Gag protein. These DCs elicited a strong HIV-1 Gag-specific interferon-gamma (IFN-gamma) response by an HLA-A2-restricted CD8+ T-cell line. Moreover, hHXB-2 gag mRNA-electroporated DCs also triggered IFN-gamma secretion by autologous peripheral blood mononuclear cells (PBMCs), CD4+ T cells, and CD8+ T cells from all patients tested. Next, a novel strategy was developed using autologous virus sequences. Significant specific IFN-gamma T-cell responses were induced in all patients tested by DCs electroporated with patients' autologous polymerase chain reaction (PCR)-amplified and in vitro-transcribed proviral and plasma viral mRNA encoding either Gag or Env. The stimulatory effect was seen on PBMCs, CD8+ T cells, and CD4+ T cells, demonstrating both major histocompatibility complex (MHC) class I and MHC class II antigen presentation. Moreover, a significant interleukin-2 (IL-2) T-cell response was induced by DCs electroporated with hHxB-2 or proviral gag mRNA. These findings open a major perspective for the development of patient-specific immunotherapy for HIV-1 disease.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-4971
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pubmed:author |
pubmed-author:AllardSabineS,
pubmed-author:BernemanZwi NZN,
pubmed-author:ColebundersRobertR,
pubmed-author:De RooAnnA,
pubmed-author:HeyndrickxLeoL,
pubmed-author:HoeffelGuillaumeG,
pubmed-author:HosmalinAnneA,
pubmed-author:MarañónConcepciónC,
pubmed-author:MoermanFilipF,
pubmed-author:PonsaertsPeterP,
pubmed-author:Van BockstaeleDirk RDR,
pubmed-author:Van GulckEllen R AER,
pubmed-author:Van TendelooViggo F IVF,
pubmed-author:Van den BoschGlennG,
pubmed-author:VanhamGuidoG,
pubmed-author:VereeckenKatleenK,
pubmed-author:VerrierBernardB
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
107
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1818-27
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16263796-Adoptive Transfer,
pubmed-meshheading:16263796-Adult,
pubmed-meshheading:16263796-CD4-Positive T-Lymphocytes,
pubmed-meshheading:16263796-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16263796-Cell Line,
pubmed-meshheading:16263796-Dendritic Cells,
pubmed-meshheading:16263796-Electroporation,
pubmed-meshheading:16263796-Female,
pubmed-meshheading:16263796-Gene Products, gag,
pubmed-meshheading:16263796-Glycoproteins,
pubmed-meshheading:16263796-HIV Seropositivity,
pubmed-meshheading:16263796-HIV-1,
pubmed-meshheading:16263796-HLA-A2 Antigen,
pubmed-meshheading:16263796-Humans,
pubmed-meshheading:16263796-Interferon-gamma,
pubmed-meshheading:16263796-Lymphocyte Activation,
pubmed-meshheading:16263796-Male,
pubmed-meshheading:16263796-Middle Aged,
pubmed-meshheading:16263796-Monocytes,
pubmed-meshheading:16263796-RNA, Viral,
pubmed-meshheading:16263796-Transplantation, Autologous,
pubmed-meshheading:16263796-Viral Envelope Proteins
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pubmed:year |
2006
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pubmed:articleTitle |
Efficient stimulation of HIV-1-specific T cells using dendritic cells electroporated with mRNA encoding autologous HIV-1 Gag and Env proteins.
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pubmed:affiliation |
HIV and Retrovirology Research Unit, Department of Microbiology, Institute of Tropical Medicine of Antwerp, Nationalestraat 155, 2000 Antwerp, Belgium. evangulck@itg.be
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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