Linked Life Data

16263129

Source:http://linkedlifedata.com/resource/pubmed/id/16263129

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2005-12-26
pubmed:abstractText
Cytokines produced by Th1 or Th2 cells have been postulated to be important in the development of type 1 diabetes in humans and animal models, such as murine multiple low-dose streptozotocin (MLDSTZ)-induced diabetes. The aim of this study was to investigate cytokine production with or without in vitro depletion of plastic adherent cells from spleens isolated after MLDSTZ treatment. Spleen cells were prepared on day 14 from MLDSTZ- and saline-treated mice and divided into two fractions. One cell fraction was depleted of adherent cells by plastic adherence and the other was not. Both cell fractions were analysed by FACS for the distribution of immune cells. In other experiments, the cells were cultured for 48 h with concanavalin A stimulation. Supernatant samples were analysed by ELISA for TNFalpha, IFNgamma and IL-10 production. Either before or after the 48-h culture cytokine mRNA expression was determined by RT-PCR. Plastic adhesion decreased the macrophage numbers by approximately 30% and CD4(+)CD25(+) cells by about 60%. This was accompanied by increased medium levels of TNFalpha, IFNgamma and IL-10, which suggest that either CD4(+)CD25(+) cells, macrophages, or both, down-regulate production of both Th1 and certain Th2 cytokines. Depletion of adherent cells also decreased IL-4 mRNA amounts. MLDSTZ treatment increased the production of Th1 cytokines mainly at the protein level, and IL-10 mainly at the mRNA level. This indicates a sustained increase in Th1 production after MLDSTZ treatment and an increase in IL-10 that might reflect an attempt to counteract the MLDSTZ-induced immune damage. Plastic adhesion during cell preparation may affect the relative distribution of certain immune cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
307
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-81
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16263129-Animals, pubmed-meshheading:16263129-B-Lymphocytes, pubmed-meshheading:16263129-Blood Glucose, pubmed-meshheading:16263129-Body Weight, pubmed-meshheading:16263129-Cell Adhesion, pubmed-meshheading:16263129-Cell Count, pubmed-meshheading:16263129-Concanavalin A, pubmed-meshheading:16263129-Cytokines, pubmed-meshheading:16263129-Diabetes Mellitus, Experimental, pubmed-meshheading:16263129-Dose-Response Relationship, Drug, pubmed-meshheading:16263129-Gene Expression, pubmed-meshheading:16263129-Interferon-gamma, pubmed-meshheading:16263129-Interleukin-10, pubmed-meshheading:16263129-Interleukin-4, pubmed-meshheading:16263129-Macrophages, pubmed-meshheading:16263129-Male, pubmed-meshheading:16263129-Mice, pubmed-meshheading:16263129-Mice, Inbred C57BL, pubmed-meshheading:16263129-Plastics, pubmed-meshheading:16263129-RNA, Messenger, pubmed-meshheading:16263129-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16263129-Spleen, pubmed-meshheading:16263129-Streptozocin, pubmed-meshheading:16263129-T-Lymphocyte Subsets, pubmed-meshheading:16263129-Th1 Cells, pubmed-meshheading:16263129-Th2 Cells, pubmed-meshheading:16263129-Tumor Necrosis Factor-alpha
pubmed:year
2005
pubmed:articleTitle
Impact of plastic adhesion in vitro on analysis of Th1 and Th2 cytokines and immune cell distribution from mice with multiple low-dose streptozotocin-induced diabetes.
pubmed:affiliation
Department of Medical Cell Biology, Biomedicum, Uppsala, Sweden. Lina.Thorvaldson@medcellbiol.uu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't