|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9-10
|
|
pubmed:dateCreated |
2005-11-1
|
|
pubmed:abstractText |
We report a novel implantable device that will deliver a tethered aligned collagen guidance conduit containing Schwann cells into a peripheral nerve injury site. Cells (Schwann cells and fibroblasts) incorporated into tethered rectangular collagen gels contracted and resulted in uniaxial alignment. This tissue-engineered construct was tested in three-dimensional culture and demonstrated the ability to guide neurite extension from dissociated dorsal root ganglia. A silicone tube was adapted to provide tethering sites for an implantable construct such that uniaxial cell-generated tension resulted in the formation of a bridge of aligned collagen fibrils, with a resident Schwann cell population. The potential of this device for surgical nerve regeneration was assessed in a 5-mm defect in a rat sciatic nerve model. Neural regeneration through this device was significantly greater than in controls, demonstrating that this system has potential both as a simple robust clinical implant and as a three-dimensional engineered tissue model.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1076-3279
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
11
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1611-7
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:16259614-Animals,
pubmed-meshheading:16259614-Biocompatible Materials,
pubmed-meshheading:16259614-Cell Culture Techniques,
pubmed-meshheading:16259614-Cells, Cultured,
pubmed-meshheading:16259614-Collagen,
pubmed-meshheading:16259614-Female,
pubmed-meshheading:16259614-Fluorescein-5-isothiocyanate,
pubmed-meshheading:16259614-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:16259614-Fluorescent Dyes,
pubmed-meshheading:16259614-Ganglia, Spinal,
pubmed-meshheading:16259614-Gels,
pubmed-meshheading:16259614-Immunohistochemistry,
pubmed-meshheading:16259614-Male,
pubmed-meshheading:16259614-Microscopy, Fluorescence,
pubmed-meshheading:16259614-Nerve Regeneration,
pubmed-meshheading:16259614-Nerve Tissue,
pubmed-meshheading:16259614-Neurites,
pubmed-meshheading:16259614-Peroneal Nerve,
pubmed-meshheading:16259614-Random Allocation,
pubmed-meshheading:16259614-Rats,
pubmed-meshheading:16259614-Rats, Inbred F344,
pubmed-meshheading:16259614-Rats, Sprague-Dawley,
pubmed-meshheading:16259614-Rhodamines,
pubmed-meshheading:16259614-S100 Proteins,
pubmed-meshheading:16259614-Schwann Cells,
pubmed-meshheading:16259614-Sciatic Nerve,
pubmed-meshheading:16259614-Silicones,
pubmed-meshheading:16259614-Tibial Nerve,
pubmed-meshheading:16259614-Time Factors,
pubmed-meshheading:16259614-Tissue Engineering,
pubmed-meshheading:16259614-Transplantation, Homologous
|
|
pubmed:articleTitle |
Neural tissue engineering: a self-organizing collagen guidance conduit.
|
|
pubmed:affiliation |
University College London, Tissue Repair and Engineering Centre, Institute of Orthopaedics and Musculoskeletal Science, Royal National Orthopaedic Hospital, Stanmore, Middlesex, UK. j.b.phillips@open.ac.uk
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
