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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-10-31
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pubmed:abstractText |
HtrA2/Omi is a mammalian mitochondrial serine protease homologous to the E. coli HtrA/DegP gene products. Recently, HtrA2/Omi was found to have a dual role in mammalian cells, acting as an apoptosis-inducing protein and being involved in maintenance of mitochondrial homeostasis. By screening a human brain cDNA library with A beta peptide as bait in a yeast two-hybrid system, we identified HtrA2/Omi as a binding partner of A beta peptide. The interaction between A beta peptide and HtrA2/Omi was confirmed by an immunoblot binding assay. The possible involvement of HtrA2/Omi in A beta peptide metabolism was investigated. In vitro peptide cleavage assays showed that HtrA2/Omi did not directly promote the production of A beta peptide at the beta/gamma-secretase level, or the degradation of A beta peptide. However, overexpression of HtrA2/Omi in K269 cells decreased the production of A beta40 and A beta42 by up to 30%. These results rule out the involvement of HtrA2/Omi in the etiology of Alzheimer's disease. However, the fact that overexpression of HtrA2/Omi reduces the generation of A beta40 and A beta42 suggests that it may play some positive role in mammalian cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1016-8478
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
83-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:16258245-Amyloid Precursor Protein Secretases,
pubmed-meshheading:16258245-Amyloid beta-Peptides,
pubmed-meshheading:16258245-Animals,
pubmed-meshheading:16258245-Apoptosis,
pubmed-meshheading:16258245-Aspartic Acid Endopeptidases,
pubmed-meshheading:16258245-Brain,
pubmed-meshheading:16258245-Cells, Cultured,
pubmed-meshheading:16258245-Dose-Response Relationship, Drug,
pubmed-meshheading:16258245-Endopeptidases,
pubmed-meshheading:16258245-Gene Library,
pubmed-meshheading:16258245-Humans,
pubmed-meshheading:16258245-Mitochondrial Proteins,
pubmed-meshheading:16258245-Serine Endopeptidases,
pubmed-meshheading:16258245-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:16258245-Transfection,
pubmed-meshheading:16258245-Two-Hybrid System Techniques
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pubmed:year |
2005
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pubmed:articleTitle |
HtrA2 interacts with A beta peptide but does not directly alter its production or degradation.
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pubmed:affiliation |
Department of Microbiology and Research Center for Industrial Development of Biofood Materials, Chonbuk National University Medical School, Jeonju 561-756, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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