| pubmed-article:16250007 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C0039421 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C0042172 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C1281743 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C0035693 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C1136031 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:16250007 | lifeskim:mentions | umls-concept:C0301625 | lld:lifeskim |
| pubmed-article:16250007 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16250007 | pubmed:dateCreated | 2005-11-30 | lld:pubmed |
| pubmed-article:16250007 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:abstractText | RNA interference (RNAi) has become acknowledged as an effective and useful tool to study gene function in diverse groups of cells. We aimed to suppress the expression of the E-cadherin gene during in vitro development of bovine preimplantation embryos using RNAi approach. In this experiment the effect of microinjection of E-cadherin and Oct-4 (as control) double-stranded (ds) RNA on the mRNA and protein expression level of the target E-cadherin gene was investigated. For this, a 496 bp long bovine E-cadherin and 341 bp long Oct-4 dsRNA sample were prepared using in vitro transcription. In vitro produced bovine zygotes were categorized into four treatment groups including those injected with E-cadherin dsRNA, Oct-4 dsRNA, RNase-free water, and uninjected controls. While the injection of E-cadherin dsRNA resulted in the reduction of E-cadherin mRNA and protein levels at the morula and blastocyst stage, the transcript and protein product remained unaffected in the Oct-4 dsRNA, water injected and uninjected control groups. The relative abundance of E-cadherin mRNA in the E-cadherin dsRNA injected morula stage embryos was reduced by 80% compared to the control group (P < 0.05). The Western blot analysis also showed a significant decrease in the E-cadherin protein (119 kDa) in E-cadherin dsRNA injected embryos compared to the other three groups. Microinjection of E-cadherin dsRNA has resulted only 22% blastocyst rate compared to 38%-40% in water injected and uninjected controls. In conclusion, our results indicated the suppression of E-cadherin mRNA and protein has resulted in lower blastocyst rate and the RNAi technology is a promising approach to study the function of genes in early bovine embryogenesis. | lld:pubmed |
| pubmed-article:16250007 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16250007 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16250007 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16250007 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16250007 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:16250007 | pubmed:issn | 1040-452X | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:SchellanderKa... | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:TesfayeDawitD | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:GillesMarkusM | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:HölkerMichael... | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:Nganvongpanit... | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:MüllerHeikeH | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:RingsFrancaF | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:JennenDanyelD | lld:pubmed |
| pubmed-article:16250007 | pubmed:author | pubmed-author:TholenErnstE | lld:pubmed |
| pubmed-article:16250007 | pubmed:copyrightInfo | (c) 2005 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:16250007 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16250007 | pubmed:volume | 73 | lld:pubmed |
| pubmed-article:16250007 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16250007 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16250007 | pubmed:pagination | 153-63 | lld:pubmed |
| pubmed-article:16250007 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16250007 | pubmed:meshHeading | pubmed-meshheading:16250007... | lld:pubmed |
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| pubmed-article:16250007 | pubmed:meshHeading | pubmed-meshheading:16250007... | lld:pubmed |
| pubmed-article:16250007 | pubmed:meshHeading | pubmed-meshheading:16250007... | lld:pubmed |
| pubmed-article:16250007 | pubmed:meshHeading | pubmed-meshheading:16250007... | lld:pubmed |
| pubmed-article:16250007 | pubmed:meshHeading | pubmed-meshheading:16250007... | lld:pubmed |
| pubmed-article:16250007 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16250007 | pubmed:articleTitle | Targeted suppression of E-cadherin gene expression in bovine preimplantation embryo by RNA interference technology using double-stranded RNA. | lld:pubmed |
| pubmed-article:16250007 | pubmed:affiliation | Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Endenicher Allee 15, Bonn, Germany. | lld:pubmed |
| pubmed-article:16250007 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16250007 | pubmed:publicationType | In Vitro | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16250007 | lld:pubmed |
